Food and Fermentation Industries >

2022 , Vol. 48 >Issue 14: 218 - 225

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028379

Analysis of eight phenolic contents in peanuts skin from different cultivars

  • GAO Jinhong ,
  • LU Xin ,
  • SUN Qiang ,
  • HUANG Jinian ,
  • WANG Qiang ,
  • JIA Cong ,
  • WANG Ruidan
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  • 1(Institute of Agricultural and Sideline Products Processing, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
    2(Research Centre for Agricultural and Sideline Products Processing, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)
    3(Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

Received date: 2021-06-15

  Revised date: 2021-07-19

  Online published: 2022-08-19

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Abstract

To investigate the difference of phenolic contents in peanuts skin from different cultivars, a HPLC method was established for simultaneous determination of 8 phenolic compounds in 40 peanuts skin varieties. Various analysis methods were used for comprehensive evaluation.The chromatographic separation was achieved using an X-Bridge shield RP18(4.6 mm×250 mm, 5 μm) through gradient elution with a the mobile phasesconsisting of acetic acid in water (volume ratio 1∶1 000, A) and acetic acid in acetonitrile (volume ratio 1∶1 000, B). The detection wavelengthswere set at 250,280, 320 and 370 nm. The results showed that 8 phenolic compounds could be separated well withgood repeatability(RSD≤3.92%) and stability (RSD≤1.96%).Statistical analysis and heat map analysis showed that there were obvious differences in 8 phenolic substances among the varieties. Correlation analysis showed that there was astrong positive correlation between protocatechuic acid, catechin, epicatechin gallate and procyanidin C1, catechin and caffeic acid. Moreover,there was astrong negative correlation between ferulic acidandthree components, including protocatechuic acid, procyanidin C1 and epicatechin gallate. Cluster analysisshowed that 40 peanut skins could be clustered into 3 categories.Therefore, there were obvious differences among the varieties.And there was a certain correlation between phenolic substances.This study could provide a theoretical reference for exploring the advantages of peanut germplasm resources in China.

Key words： peanut skin; phenolic compounds; heatmap analysis; correlation analysis; cluster analysis

Cite this article

GAO Jinhong , LU Xin , SUN Qiang , HUANG Jinian , WANG Qiang , JIA Cong , WANG Ruidan . Analysis of eight phenolic contents in peanuts skin from different cultivars[J]. Food and Fermentation Industries, 2022 , 48(14) : 218 -225 . DOI: 10.13995/j.cnki.11-1802/ts.028379

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