To explore the effective dispersion method of cell aggregates in aqueous solution system, Saccharomyces cerevisiae ATCC 9080 was used as the target strain. The effects of tween 20, ethylene diamine tetra acetic acid (EDTA), ionic strength, pH and low-frequency ultrasound (50 Hz) on its cell dispersion index were researched. Then orthogonal test was designed on tween 20, EDTA and ultrasound to obtain the optimized dispersion condition, and the distribution types of S. cerevisiae was also analyzed before and after dispersion treatment. The results showed that the optimal protocol for the dispersion of S. cerevisiae was under the condition of 0.2% tween 20, 8 mmol/L EDTA and ultrasonicating for 15 s in PBS, which could increase the dispersion index to 0.24 in aqueous solution. The distribution type of cells was changed from cluster distribution of control group to regular distribution. Moreover, there was no effect on the viability of S. cerevisiae under the optimal dispersion conditions.
CHEN Xiangyu
,
LI Xuesong
,
XU Juan
,
LI Mengmeng
,
LIN Lijun
,
LU Lixia
,
LIU Yuanjian
,
XIONG Xiaohui
. Study on promoting the dispersion of Saccharomyces cerevisiae in aqueous solution system[J]. Food and Fermentation Industries, 2022
, 48(15)
: 102
-109
.
DOI: 10.13995/j.cnki.11-1802/ts.031130
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