To explore the best tenderization process of pig large intestine, the effects of NaHCO3, NaOH, CaCl2, papain and bromelain on pig large intestine tenderization were studied. The results showed that NaOH and papain had significant(P<0.05) tenderization effects on pig large intestine. Then, based on the single factor, the effects of various factors on the quality of pig large intestine were studied. Taking the sensory score and shear force of pig large intestine as the response value, according to the principle of response surface experimental design, the multiple quadratic regression equation was established. When NaOH was used as tenderizing agent, the response surface results showed that the treatment time had the most significant(P<0.01) effect on the quality of pig large intestine. The best parameter combination was 1.1 g/L NaOH at the treatment of 41 ℃ for 24 min. Meanwhile, the sensory score of the pig large intestine was 7.7, which was basically consistent with the predicted value of the model. When papain was used as tenderizing agent, the response surface results showed that the treatment time had the most significant (P<0.01) effect on the quality of tripe. The best parameter combination was 2.0% of papain at the temperature of 36 ℃for 102 min. At this time, the shear force of the pig large intestine was 11.70 N, which was basically consistent with the predicted value of the model.
CHENG Chengpeng
,
HE Zhifei
,
TANG Chun
,
LI Fang
,
LIAO Lin
,
LI Hongjun
. Process optimization for the improvement of pig large intestine tenderness with different tenderizing agents[J]. Food and Fermentation Industries, 2022
, 48(15)
: 249
-256
.
DOI: 10.13995/j.cnki.11-1802/ts.029532
[1] 刘丹丹, 闫佳旭, 李瑞, 等.猪大肠的养生价值探讨[J].安徽农业科学, 2013, 41(13):5 753-5 754;5 775.
LIU D D, YAN J X, LI R, et al.The brief discussion on health value of pig intestine[J].Journal of Anhui Agricultural Sciences, 2013, 41(13):5 753-5 754;5 775.
[2] AASLYNG M D, MEINERT L.Meat flavour in pork and beef-From animal to meal[J].Meat Science, 2017, 132:112-117.
[3] PICARD B, GAGAOUA M.Proteomic investigations of beef tenderness[J]. Proteomics in Food Science, 2017:177-197.
[4] 徐秋良, 吴运香, 张长兴, 等.畜禽肉嫩度及其影响因素[J].家畜生态学报, 2010, 31(6):100-103.
XU Q L, WU Y X, ZHANG C X, et al.Animal meat tenderness and its influencing factors[J].Acta Ecologiae Animalis Domastici, 2010, 31(6):100-103.
[5] OBUZ E, AKKAYA L, GÖK V, et al.Effects of blade tenderization, aging method and aging time on meat quality characteristics of Longissimus lumborum steaks from cull Holstein cows[J].Meat Science, 2014, 96(3):1 227-1 232.
[6] BALASUBRAMANIAM V M B, MARTÍNEZ-MONTEAGUDO S I, GUPTA R.Principles and application of high pressure-based technologies in the food industry[J].Annual Review of Food Science and Technology, 2015, 6:435-462.
[7] ENG L Y, HE Z F, LI H J.Research progress on the application of shockwave to tenderize meat[J].Food and Fermentation Industries, 2019, 45(5):259-265.
[8] BERGE P, ERTBJERG P, LARSEN L M, et al.Tenderization of beef by lactic acid injected at different times post mortem[J].Meat Science, 2001, 57(4):347-357.
[9] GRZONKA Z, KASPRZYKOWSKI F, WICZK W.Cysteine Proteases[M]//Industrial Enzymes.Dordrecht:Springer Netherlands, 2007.
[10] SANGEETHA K, ABRAHAM T E.Chemical modification of papain for use in alkaline medium[J].Journal of Molecular Catalysis B:Enzymatic, 2006, 38(3-6):171-177.
[11] LEE W C, CHEN T C.Functional characteristics of egg white solids obtained from papain treated albumen[J].Journal of Food Engineering, 2002, 51(4):263-266.
[12] DJUKIĆ-VUKOVIĆ A P, MOJOVIĆ L V, VUKAŠINOVIĆ-SEKULIĆ M S, et al.Effect of different fermentation parameters on L-lactic acid production from liquid distillery stillage[J].Food Chemistry, 2012, 134(2):1 038-1 043.
[13] 李明奇, 贺稚非, 李少博, 等.氯化钙-无花果蛋白酶-猕猴桃蛋白酶复合嫩化剂体系改善兔肉嫩度和保水性的工艺优化[J].食品与发酵工业, 2019, 45(18):120-129.
LI M Q, HE Z F, LI S B, et al.Optimization of calcium chloride, ficin and kiwifruit protease tenderization system to improve the tenderness and water holding capability of rabbit meat[J].Food and Fermentation Industries, 2019, 45(18):120-129.
[14] 王兆明. 兔肉滚揉腌制工艺及品质特性变化研究[D].重庆:西南大学, 2016.
WANG Z M.Research on tumbling process and quality changes of rabbit meat[D].Chongqing:Southwest University, 2016.
[15] SALLAM K I.Chemical, sensory and shelf life evaluation of sliced salmon treated with salts of organic acids[J].Food Chemistry, 2007, 101(2):592-600.
[16] 胡代芳, 李洪军, 贺稚非.响应面试验优化毛肚碱发工艺[J].食品与发酵工业, 2018, 44(8):211-217.
HU D F, LI H J, HE Z F.Optimization of lye macerating process of beef tripe by response surface methodology[J].Food and Fermentation Industries, 2018, 44(8):211-217.
[17] 杨鸿基, 韩玲, 孔祥颖, 等.氯化钙-木瓜蛋白酶-碳酸氢钠嫩化体系改善原切牦牛排嫩度[J].食品科学, 2019, 40(20):261-268.
YANG H J, HAN L, KONG X Y, et al.Using a mixed system of calcium chloride, papain and sodium bicarbonate to improve the tenderness of conventional cut yak steak[J].Food Science, 2019, 40(20):261-268.
[18] HUFF-LONERGAN E, LONERGAN S M.Mechanisms of water-holding capacity of meat:The role of postmortem biochemical and structural changes[J].Meat Science, 2005, 71(1):194-204.
[19] ZHANG N, JI M, PAN T, et al.Application of Box-Behnken design in optimization of allelopathic effects of Potamogeton pectinatus against Microcystis aeruginosa[J].Transactions of Tianjin University, 2014, 20(5):344-349.
[20] 陈云川. 碱发机理再探[J].食品科学, 1990,11(5):17-19.
CHEN Y C.Study on the mechanism of alkaline precipitation[J].Food Science, 1990,11(5):17-19.
[21] 陈洪. 碱发机理研究[J].食品科学, 1988, 9(10):17-19.
CHEN H.Further exploration of alkali generating mechanism[J].Food Science, 1988, 9(10):17-19.
[22] 汪洋, 王稳航.肌内结缔组织与肉嫩度的关联机制及相关肉嫩化技术的研究进展[J].食品科学, 2021, 42(11):332-340.
WANG Y, WANG W H.A review of the mechanism of the association between intramuscular connective tissue and meat tenderness and related meat tenderization technologies[J].Food Science, 2021, 42(11):332-340.