Food and Fermentation Industries >

2022 , Vol. 48 >Issue 16: 11 - 17

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031344

Effect of Lactobacillus reuteri CCFM8631 on choline-induced elevation of plasma trimethylamine-N-oxide and cecal trimethylamine levels

  • WANG Qianqian ,
  • ZHANG Ting ,
  • LIANG Ming ,
  • ZHAO Jianxin ,
  • ZHANG Hao ,
  • WANG Gang ,
  • CHEN Wei
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  • 1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(Infinitus (China) Company Ltd., Guangzhou 510645, China)

Received date: 2022-03-01

  Revised date: 2022-03-17

  Online published: 2022-09-16

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Abstract

Cardiovascular diseases have the highest mortality and morbidity rates in the world, for which atherosclerosis (AS) is the main pathologic basis. AS occurrence and development are related to vascular inflammation, hypercholesterolemia and oxidative stress. In recent years, researchers have found that gut microbiota play a key role in the occurrence and development of AS. High plasma trimethylamine-N-oxide (TMAO) concentrations, mainly from choline metabolite by gut microbiota or from fish intake, have been found to be an independent risk factor for promoting AS. TMAO promotes AS occurrence and development in several ways. Ingesting dietary components that reduce TMAO production may be a promising strategy by which to prevent AS. Given the role of gut microbiota in the pathogenesis of TMAO-induced AS, gut microbiota offers a potential therapeutic target for preventing and treating cardiovascular disease. Dietary composition affects the community structure of intestinal microorganisms and gut microbiota quickly adapt to dietary changes. In the light of growing evidence of the relationship between gut microbiota and chronic diseases, probiotics have received increasing attention as intervention targets. Probiotics show specificity and functionality for alleviating chronic diseases and reducing inflammation, and preclinical studies have been demonstrated to relieve liver damage, reduce weight and remit colitis. In order to analyze the ability of Lactobacillus to reduce TMAO, in this study, three different strains of Lactobacillus were given choline-fed mice by gavage. The levels of plasma TMAO and cecal trimethylamine (TMA) in mice were detected by liquid chromatography-mass spectrometry (HPLC-MS/MS), and the diversity and species composition of mouse fecal microbiota were analyzed by Illumina MiSeq high-throughput sequencing technology. It was found that Lactobacillus reuteri CCFM8631 could significantly reduce the contents of plasma TMAO and cecal TMA. This reduction was not achieved by affecting the expression of hepatic FMO3 and FXR proteins and the enzymatic activity of hepatic FMOs, but is related to the regulation of gut microbiota by lactic acid bacteria.

Key words： gut microbiota; trimethylamine-N-oxide; trimethylamine; atherosclerosis; Lactobacillus reuteri

Cite this article

WANG Qianqian , ZHANG Ting , LIANG Ming , ZHAO Jianxin , ZHANG Hao , WANG Gang , CHEN Wei . Effect of Lactobacillus reuteri CCFM8631 on choline-induced elevation of plasma trimethylamine-N-oxide and cecal trimethylamine levels[J]. Food and Fermentation Industries, 2022 , 48(16) : 11 -17 . DOI: 10.13995/j.cnki.11-1802/ts.031344

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