Food and Fermentation Industries >

2022 , Vol. 48 >Issue 16: 42 - 49

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030048

Preliminary study of terpenes based on autophagy

  • LI Yishu ,
  • YAO Yiping ,
  • HUANG Heqiang ,
  • CHEN Shanbin ,
  • ZHAO Wenmei ,
  • YANG Haicun ,
  • WANG Deliang ,
  • HAO Feike
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  • 1(College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China)
    2(China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing 100015, China)
    3(International Joint Research Center of Quality and Safety of Alcoholic Beverages, Beijing 100015, China)
    4(Qinghai Huzhu Barley Wine Co.Ltd., Haining 810500, China)

Received date: 2021-11-11

  Revised date: 2022-01-04

  Online published: 2022-09-16

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Abstract

By treating cells with different concentrations of terpene compounds, we investigated whether the seven terpene compounds contained in highland barley wine will affect autophagy. Control group and terpene compound treatment group with different mass concentrations were set up for LO2 cells. CCK-8 method was used to detect cell viability; western blotting was used to detect the expression of microtubule-associated proteins 1A/1B light chain 3 (LC3) in LO2 cells; real-time quantitative polymer chain reaction (RT-qPCR) was used to detect the mRNA expression level of LC3 and P62/SQSTM1 protein; fluorescence immunoassay was used to observe the expression level of LC3 and P62 protein. Results showed that under nutrient and starvation conditions, the LC3 protein expression level and mRNA expression level after α-pinene treatment were up-regulated in a concentration-dependent manner, while the mRNA expression level of P62 decreased. The remaining six terpene compounds, citronellol, β-caryophyllene, myrcene, ocimene, limonene, and β-ionone had no significant effect on autophagy. In LO2 cells, the terpenoid α-pinene may have the effect of promoting autophagy, and its specific mechanism still needs to be studied in depth.

Key words： terpenes; α-pinene; cell autophagy; LO2 cells

Cite this article

LI Yishu , YAO Yiping , HUANG Heqiang , CHEN Shanbin , ZHAO Wenmei , YANG Haicun , WANG Deliang , HAO Feike . Preliminary study of terpenes based on autophagy[J]. Food and Fermentation Industries, 2022 , 48(16) : 42 -49 . DOI: 10.13995/j.cnki.11-1802/ts.030048

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