In order to improve the tenderness and water retention of pig large intestine, the enzyme-alkali combined tenderization was used to tenderize pig large intestine. Taking the shear force and cooking loss as indexes, the tenderization effects of different tenderization processes on pig large intestine were studied. The results showed that the combined enzyme-alkali process had the most obvious effect on pig large intestine tenderization. Then, taking yield, cooking loss and shear force as evaluation indexes, single factor experiments were carried out to study the effects of different process conditions on the tenderization of pig large intestine. On the basis of single factor, according to the experimental design principle of response surface, the training, test and validation data required by BP neural network training model were obtained. Genetic algorithm was used to optimize the process conditions of enzyme-alkali combined tenderization. The results showed that the process parameters optimized by BP neural network combined with genetic algorithm were as follows: NaOH 1.40 g/L, NaOH treatment time 26 min, papain mass fraction 2.0 %, papain treatment time 55 min, and the sensory score of pig large intestine reached 9.125, which was basically consistent with the predicted value of the model. The tenderization process significantly improved the tenderness and water retention of pig large intestine, and had a certain reference value for the processing of pig large intestine.
CHENG Chengpeng
,
HE Zhifei
,
TANG Chun
,
LIU Shuyun
,
XIAO Xu
,
LI Hongjun
. Optimization of enzyme-alkali combined process for improving tenderness and water retention of pig large intestine[J]. Food and Fermentation Industries, 2022
, 48(16)
: 87
-94
.
DOI: 10.13995/j.cnki.11-1802/ts.030254
[1] 王复龙, 高菲菲, 彭增起.pH变换腌制对牛胃平滑肌嫩度、保水性及微观结构的影响[J].食品工业科技, 2016,37(3):110-113;118.
WANG F L,GAO F F,PENG Z Q.Effect of pH shifting marinating on the tenderness,water retention and microstructure in cattle gastric smooth muscle[J].Science and Technology of Food Industry, 2016,37(3):110-113;118.
[2] 孙溥泉, 孙健慧.猪肾的营养、药膳及验方[J].养猪, 2012(1):79-80.
SUN P Q, SUN J H.Nutrition, medicinal diet and prescription of pig intestines[J].Swine Production, 2012(1):79-80.
[3] AASLYNG M D, MEINERT L.Meat flavour in pork and beef-From animal to meal[J].Meat Science, 2017,132:112-117.
[4] 郎玉苗. 肌纤维类型对牛肉嫩度的影响机制研究[D].北京:中国农业科学院, 2016.
LANG Y M.Mechanism and effects of muscle fiber types on beef tenderness[D].Beijing:Chinese Academy of Agricultural Sciences, 2016.
[5] 常海军. 不同加工条件下牛肉肌内胶原蛋白特性变化及其对品质影响研究[D].南京:南京农业大学, 2010.
CHANG H J.Studies on effects of different processing conditions on characteristics changes of collagen and meat quality of beef muscle.[D].Nanjing:Nanjing Agricultural University, 2010.
[6] 郝婉名, 祝超智, 赵改名, 等. 西门塔尔杂交牛不同部位肉间的差异性[J]. 肉类研究, 2019, 33(1):14-18.
HAO W M, ZHU C Z, ZHAO G M, et al. Differences in meat quality among different parts of crossbred Simmental cattle[J]. Meat Research, 2019, 33(1):14-18.
[7] LISTRAT A, GAGAOUA M, NORMAND J, et al.Contribution of connective tissue components, muscle fibres and marbling to beef tenderness variability in longissimus thoracis, rectus abdominis, semimembranosus and semitendinosus muscles[J].Journal of the Science of Food and Agriculture, 2020,100(6):2 502-2 511.
[8] MARINO R, DELLA MALVA A, ALBENZIO M.Proteolytic changes of myofibrillar proteins in Podolian meat during aging:focusing on tenderness[J].Journal of Animal Science, 2015,93(3):1 376-1 387.
[9] KIM H W, CHOI Y S, CHOI J H, et al.Tenderization effect of soy sauce on beef M.biceps femoris[J].Food Chemistry, 2013,139(1-4):597-603.
[10] 张坤, 王道营, 张淼, 等.高强度超声对鹅胸肉嫩度及品质的影响[J].食品科学, 2018,39(15):122-127.
ZHANG K, WANG D Y, ZHANG M, et al.Effect of high-intensity ultrasonic treatment on goose tenderness and meat quality[J].Food Science, 2018,39(15):122-127.
[11] PURCHAS R W.An assessment of the role of pH differences in determining the relative tenderness of meat from bulls and steers.[J].Meat Science, 1990,27(2):129-140.
[12] DEMAN, JOHN M.Principles of Food Chemistry[M]. Dordrecht: Kluwer Academic Pub,1995.
[13] 李鹏. 不同极限pH值牛肉成热过程中品质变化及变化机制研究[D].泰安:山东农业大学, 2014.
LI P.Quality changes and underlying mechanisms of different ultimate pH beef during ageing[D].Taian:Shandong Agricultural University, 2014.
[14] 白玲燕. 木瓜蛋白酶处理对牛肉品质影响的研究[J].食品安全导刊, 2021(26):122-124.
BAI L Y.Effect of papain treatment on beef quality[J].China Food Safety Magazine, 2021(26):122-124.
[15] NAVEENA B M, MENDIRATTA S K, ANJANEYULU A S R.Tenderization of buffalo meat using plant proteases from Cucumis trigonus Roxb (Kachri) and Zingiber officinale roscoe (Ginger rhizome)[J].Meat Science, 2004,68(3):363-369.
[16] 李明奇. 复合嫩化剂对兔肉理化特性及贮藏品质的影响[D].重庆:西南大学, 2020.
LI M Q.Effect of compound tenderizer on physical and chemical properties and storage quality of rabbit meat[D].Chongqing:Southwest University, 2020.
[17] 王兆明. 兔肉滚揉腌制工艺及品质特性变化研究[D].重庆:西南大学, 2016.
ZHANG Z M.Research on tumbling process and quality changes of rabbit meat[D].Chongqing:Southwest University, 2016.
[18] 张斌, 孙兰萍, 胡海燕, 等.基于模糊数学和响应面法的超高压嫩化河蚌肉的感官评价[J].食品与发酵工业, 2017,43(6):157-162.
ZHANG B,SUN L P,HU H Y, et al.The sensory evaluation of mussel meat tenderization treated by ultra-high pressure based on fuzzy mathematics and response surface method[J].Food and Fermentation Industries, 2017,43(6):157-162.
[19] HAYKIN S.Neural Networks:A Comprehensive Foundation (3rd Edition)[M].Neural Networks:A Comprehensive Foundation (3rd Edition), 1998.
[20] 甜甜, 张松山, 雷元华, 等.碱-酶联合脱毛法对牛皮营养成分与品质的影响[J].食品工业科技, 2020,41(18):170-175.
TIAN T,ZHANG S S,LEI Y H, et al.Effects of alkali-enzyme combined with dehairing methods on the nutrients and quality of cowhide [J].Science and Technology of Food Industry, 2020,41(18):170-175.
[21] HUFF-LONERGAN E, LONERGAN S M.Mechanisms of water-holding capacity of meat:The role of postmortem biochemical and structural changes[J].Meat Science, 2005,71(1):194-204.
[22] KOOHMARAIE M.Biochemical factors regulating the toughening and tenderization processes of meat[J].Meat Science, 1996,43(1):193-201.
[23] 黄彩燕, 张松山, 韩玲, 等.植物蛋白酶在肉类嫩化中的应用研究进展[J].食品与发酵科技, 2017,53(4):88-91;119.
HUANG C Y, ZHANG S S, HAN L, et al.Research progress on application of plant proteinase in meat tenderization[J].Food and Fermentation Sciences & Technology, 2017,53(4):88-91;119.
[24] 姬云云, 田洪磊, 詹萍, 等.BP神经网络结合遗传算法优化羊肉汤中香辛料的添加量[J].中国食品学报, 2021,21(3):128-137.
JI Y Y, TIAN H L, ZHAN P, et al.Optimizing of the amount of spices in stewed mutton soup using BP neural network and genetic algorithm[J].Journal of Chinese Institute of Food Science and Technology, 2021,21(3):128-137.