Isolation and identification of probiotic lactic acid bacteria Weissella hellenica L-1 and its fermentation characteristics of kelp

  • GOU Zhongjun ,
  • HUANG Qiao ,
  • QU Mingcheng ,
  • ZHANG Yuhao ,
  • LI jie ,
  • XIAO Lin
Expand
  • 1(Juhui Food Technology (Chongqing) Co.Ltd., Chongqing 401147, China)
    2(College of Food Science, Southwest University, Chongqing 400715)
    3(Chongqing Food Industry Institute, Chongqing 400000, China

Received date: 2021-11-27

  Revised date: 2021-12-24

  Online published: 2022-09-16

Abstract

An increasing trend of consumption of nutrient-rich seaweeds and fermented nondairy foods arised among health-minded consumers. Research and development of new lacto-fermented seaweed products might fulfil their request on healthy food. A dominant lactic acid bacteria strain was isolated and screened from typical pickles in southwest China in this study. It was identified as Weissella hellenica by the characteristics of morphology, physiology, biochemistry and 16S rDNA sequence analysis. Acid production capacity and its growth under different salt concentration and pH stress were analyzed. Its potential of co-fermentation of kelp with Lactobacillus plantarum (homo-lactic-acid bacterium) was also comprehensively evaluated. The results showed that their co-fermentation rapidly increased the content of lactic acid bacteria, and reduced the total content of sugar and reducing sugar in kelp, which demonstrated their capacity in improving the overall flavor of kelp juice. The overall preference of consumers was influenced by the kelp content, under the kelp content of 25%, the new fermented seaweed product had an acceptable appearance. The co-fermentation sample was significantly superior to the single-strain fermentation sample in terms of kelp flavor (7.5) and compound flavor (8.9), with better desirable scent and harmonious taste. This study provides technical support and theoretical basis to the direct vat set of lactic acid bacteria in the fermentation of seaweed flavoring products.

Cite this article

GOU Zhongjun , HUANG Qiao , QU Mingcheng , ZHANG Yuhao , LI jie , XIAO Lin . Isolation and identification of probiotic lactic acid bacteria Weissella hellenica L-1 and its fermentation characteristics of kelp[J]. Food and Fermentation Industries, 2022 , 48(16) : 111 -116 . DOI: 10.13995/j.cnki.11-1802/ts.030155

References

[1] JESUMANI V, DU H, ASLAM M, et al.Potential use of seaweed bioactive compounds in skincare-A review[J].Marine Drugs,2019,17(12):688.
[2] DEL RO P G, GOMES-DIAS J S, ROCHA C M R, et al.Recent trends on seaweed fractionation for liquid biofuels production[J].Bioresource Technology, 2020, 299:122613.
[3] DE JESUS RAPOSO M F, DE MORAIS A M M B, DE MORAIS R M.Emergent sources of prebiotics:Seaweeds and microalgae[J].Marine Drugs, 2016, 14(2):27.
[4] LI N N, FU X D, XIAO M S, et al.Enzymatic preparation of a low-molecular-weight polysaccharide rich in uronic acid from the seaweed Laminaria japonica and evaluation of its hypolipidemic effect in mice[J].Food & Function,2020,11(3):2 395-2 405.
[5] ZENG J, AN D, JIAO C, et al.Cloning, expression, and characterization of a new pH-and heat-stable alginate lyase from Pseudoalteromonas carrageenovora ASY5[J]. Journal of Food Biochemistry, 2019, 43(7): e12886.
[6] MOERDIJK-POORTVLIET T C W, DE JONG D L C, FREMOUW R, et al.Extraction and analysis of free amino acids and 5′-nucleotides, the key contributors to the umami taste of seaweed[J].Food Chemistry,2022, 370:131352.
[7] S′CIESZKA S, KLEWICKA E.Algae in food:A general review[J].Critical Reviews in Food Science and Nutrition, 2019, 59(21):3 538-3 547.
[8] YAN F, POLK D B.Probiotics and probiotic-derived functional factors-mechanistic insights into applications for intestinal homeostasis[J].Frontiers in Immunology, 2020, 11:1428.
[9] XINOG T, LI J B, LIANG F, et al.Effects of salt concentration on Chinese sauerkraut fermentation[J].LWT-Food Science and Technology, 2016, 69:169-174.
[10] 张楠笛, 祝林, 许琴, 等.食窦魏斯氏菌协同植物乳杆菌改善四川泡菜风味[J].食品科学, 2020, 41(14):102-108.
ZHANG N D, ZHU L, XU Q, et al.Improvement of the flavor of Sichuan pickle by co-fermentation with Weissella cibaria and Lactobacillus plantarum[J].Food Science, 2020, 41(14):102-108.
[11] 东秀珠, 蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社, 2001.
DONG X Z, CAI M Y.Manual of Identification of Common Bacterial Systems [M].Beijing:Science Press, 2001.
[12] 王雪艳. 西藏高海拔地区酸奶中乳酸菌分离鉴定及其产酸能力评价[D].拉萨:西藏大学, 2016.
WANG X Y.Isolate and authenticate lactic acid bacteria from the yogurt of Tibet’s high altitude regions and evaluate their acid-producing ability[D].Lasa:Tibet University, 2016.
[13] JIANG Y P, QI X H, GAO K, et al.Relationship between molecular weight, monosaccharide composition and immunobiologic activity of Astragalus Polysaccharides[J]. Glycoconjugate Journal,2016,33(5):755-761.
[14] DUBOIS M, GILLES K A, HAMILTON J K, et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry, 1956, 28(3):350-356.
[15] 杨杨, 石超, 郭旭生.高寒草甸魏斯氏乳酸菌的分离鉴定及理化特性研究[J].草业学报,2014,23(1):266-275.
YANG Y, SHI C, GUO X S.Characterization and identification of Weissella species isolated from Kobresia littledalei growing in alpine meadows[J].Acta Prataculturae Sinica, 2014, 23(1):266-275.
[16] UUH-NARVAEZ J J, SEGURA-CAMPOS M R.Cabbage (Brassica oleracea var.capitata):A food with functional properties aimed to type 2 diabetes prevention and management[J].Journal of Food Science, 2021,86(11):4 775-4 798.
[17] BRUHN A, BRYNNING G, JOHANSEN A, et al.Fermentation of sugar kelp (Saccharina latissima)-effects on sensory properties, and content of minerals and metals[J].Journal of Applied Phycology, 2019, 31(5):3 175-3 187.
[18] SKONBERG D I, FADER S, PERKINS L B, et al.Lactic acid fermentation in the development of a seaweed sauerkraut-style product:Microbiological, physicochemical, and sensory evaluation[J].Journal of Food Science, 2021,86(2):334-342.
Outlines

/