Pueraria lobata and Hovenia dulcis were used as the main raw material, and the mixed fermentation of Lactobacillus plantarum and Lactobacillus paracasei were used to study the effect of the drug before and after fermentation and its anti-alcohol and liver protection effects. Through single factor test and orthogonal test using multi-index comprehensive weighted scoring method for flavonoid content and sobering time, the optimal fermentation process conditions were determined as, pH 7, total inoculation amount 2%, inoculation ratio 2∶1, and fermentation time 36 h. A mouse model of acute alcoholic liver injury was established, aspartate aminotransferase (AST), glutamate-pyruvate aminotransferase (ALT), interleukin-6 (IL-6), tumor necrosis factors (TNF-α) in the serum and the level of superoxide dismutase (SOD) in liver tissues was evaluated to determine the effect of Pueraria lobata-Hovenia dulcis mixed fermentation on alleviate hangover under the optimal conditions. The results showed that the extract of Pueraria lobata-Hovenia dulcis by mixed fermentation of L. plantarum and L. paracasei increased the content of flavonoids by 33%, prolonged the intoxication latency by 51.7%, and shorten the recovery time of righting reflex by 37.1%, significantly reduced the serum levels of IL-6, TNF-α and the enzyme activities of AST and ALT in the modeled mice, and significantly increased the activity of SOD antioxidant enzymes. The effect was better than that before fermentation. Studies confirmed that the mixed fermentation of L. plantarum and L. paracasei increased the antioxidant activity of Pueraria lobata-Hovenia dulcis, and enhanced the anti-alcoholic effect, which was better than that of unfermented drugs, and they all had a certain protective effect on alcoholic liver injury in mice.
[1] 付旭冉, 叶永丽, 赵晓联, 等.葛根功效活性及其在食品中的应用进展[J].食品研究与开发, 2021, 42(13):197-205.
FU X R, YE Y L, ZHAO X L, et al.Research progress into multiple uses for Puerariae lobatae and its application in food[J].Food Research and Development, 2021,42 (13):197-205.
[2] 许皖. 葛花、枳椇子及其配伍对急性酒精性肝损伤小鼠保护作用的实验研究[D].北京:北京中医药大学, 2020.
XU W.Experimental study on protective effects of Pueraria lobata, trifoliate orange fruit and their compatibility on acute alcoholic liver injury in mice[D].Beijing:Beijing University of Traditional Chinese Medicine, 2020.
[3] CHEN X,HE Z Y,WU X Y, et al.Comprehensive study of the interaction between Puerariae radix flavonoids and DNA:From theoretical simulation to structural analysis to functional analysis[J].Spectrochimica Acta. Part A,Molecular and Biomolecular Spectroscopy, 2020, 231:118109.
[4] LIU Y S,YUAN M H,ZHANG C Y, et al.Puerariae Lobatae radix flavonoids and puerarin alleviate alcoholic liver injury in zebrafish by regulating alcohol and lipid metabolism[J].Biomedicine & Pharmacotherapy, 2021, 134(1):111121.
[5] 管咏梅, 许攀, 沈倩, 等.葛根解酒的研究进展[J].中国实验方剂学杂志, 2021, 27(2):210-217.
GUAN Y M, XU P, SHEN Q, et al.Research progress in anti-alcoholic effect of Puerariae lobatae Radix[J].Chinese Journal of Experimental Traditional Medical Formulae, 2021, 27 (2):210-217.
[6] 宋莎莎, 路景涛, 袁平凡, 等.复方枳椇子制剂对小鼠酒精中毒的解救作用[J].中国新药杂志, 2013, 22(21):2 524-2 528.
SONG S S, LU J T, YUAN P F, et al.Detoxication effect of compound preparation of Hovenia dulcis Thunb in mice with alcoholism[J].Chinese Journal of New Drugs, 2013, 22(21):2 524-2 528.
[7] 彭景华, 崔团, 冯琴.葛根及其主要异黄酮成分解酒药理研究进展[J].中草药, 2009, 40(S1):66-69.
PENG J H, CUI T, FENG Q.Progress of pharmacological research on Pueraria lobata and its major isoflavones into alcoholic beverages[J].Chinese Traditional and Herbal Drugs, 2009, 40(S1):66-69.
[8] 王静涵, 张斯童, 滕利荣, 等.益生菌发酵中药的研究现状及产品开发[J].食品工业科技, 2020, 41(14):337-343;348.
WANG J H, ZHANG S T, TENG L R, et al.Research status and product development of probiotic fermentation of traditional Chinese medicine[J].Science and Technology of Food Industry, 2020, 41(14):337-343;348.
[9] LI L, WANG L, FAN W X, et al.The application of fermentation technology in traditional chinese medicine:A review[J].The American Journal of Chinese Medicine, 2020, 48(4):899-912.
[10] 陈艳艳, 于波, 潘黛安, 等.乳杆菌发酵葛根水提液工艺研究及其解酒功效探讨[J].中国酿造, 2020, 39(8):182-186.
CHEN Y Y, YU B, PAN D A, et al.Fermentation technology of water extract of Pueraria lobata by Lactobacillus and its alleviating hangover effect[J].China Brewing, 2020, 39(8):182-186.
[11] 肖琼, 沈平孃.中药醇沉工艺的关键影响因素[J].中成药, 2005,27(2):143-144.
XIAO Q, SHEN P N.Key influencing factors of alcohol precipitation process of traditional Chinese medicine[J].Chinese Traditional Patent Medicine, 2005,27(2):143-144.
[12] HE Y,XIN H L,HUANG K, et al.Effect of alcohol concentration in water extraction and alcohol precipitation on preparation of effervescent tablets of Banlangen[J].China Journal of Chinese Materia Medica, 2010, 35(3):288-292.
[13] 黄一挚, 赵斌斌, 盘雯慧, 等.正交试验优化安肠液的水提及醇沉工艺[J].中国医药导刊, 2021, 23(10):748-753.
HUANG Y Z, ZHAO B B, PAN W H,et al.Optimization of water extraction and ethanol precipitation processes for anchang liquid by orthogonal design[J].Chinese Journal of Medicinal Guide, 2021,23(10):748-753.
[14] XIE L,HUANG W G,LI J L, et al.The protective effects and mechanisms of modified Lvdou Gancao Decoction on acute alcohol intoxication in mice[J].Journal of Ethnopharmacology, 2022,282:114593.
[15] CHA J Y, JEONG J J, YANG H J, et al.Effect of fermented sea tangle on the alcohol dehydrogenase and acetaldehyde dehydrogenase in Saccharomyces cerevisiae[J].Journal of Microbiology and Biotechnology, 2011, 21(8):791-795.
[16] 朱振元,薛婧,刘晓翠, 等.葛根素及葛根异黄酮对小鼠急性醉酒预防和解酒效果的研究[J].食品科学, 2014, 35(15):247-250.
ZHU Z Y, XUE J, LIU X C, et al.Puerarin and isoflavones from kudzu root (Pueraria lobata Ohwi) prevent acute drunkenness and relieve alcoholism in mice[J].Food Science, 2014, 35(15):247-250.
[17] 罗安玲. 葛根、藤茶、玉米低聚肽复合组方对小鼠的解酒保肝作用研究[D].上海:上海交通大学, 2019.
LUO A L.Study on antialcoholic and hepatoprotective effect of compound of Pueraria, Ampelopsis grossedentata and corn oligopeptides against alcoholic liver injury in mice[D].Shanghai:Shanghai Jiaotong University, 2019.
[18] 王富江, 屠鹏飞, 曾克武, 等.荒漠肉苁蓉总苷对酒精性肝损伤小鼠的保护作用研究[J].药学学报, 2021, 56(9):2 528-2 535.
WANG F J, TU P F, ZENG K W, et al.Protective effects of the total glycosides of Cistanche deserticola Y.C.Ma in alcoholic liver injury in mice[J].Acta Pharmaceutica Sinica, 2021, 56 (9):2 528-2 535.
[19] DONG M J, LI L S, LI G W, et al.Mangiferin protects against alcoholic liver injury via suppression of inflammation-induced adipose hyperlipolysis[J].Food & Function, 2020, 11(10):8 837-8 851.
[20] 田慧, 韦林利, 曾昭清, 等.多指标综合加权评分法优选十一方药酒渗漉提取工艺研究[J].中药材, 2021,44(5):1 199-1 201.
TIAN H, WEI L L, ZENG Z Q, et al.Optimization of percolation extraction process of eleven prescriptions medicinal wine by multi index comprehensive weighted scoring method[J].Journal of Chinese Medicinal Materials, 2021, 44(5):1 199-1 201.
