To investigate the effects of deionized water, cola and Xinjiang sea buckthorn juice on the structure of enamel. Fifteen bovine maxillary incisors were collected, and the crowns of the bovine incisors were prepared into enamel blocks of 5 mm × 5 mm × 2 mm size. Using a random number table, 30 samples were randomly divided into cola, deionized water and Xinjiang sea buckthorn juice groups, with 10 enamel blocks in each group. The enamel blocks were all placed in a 37°C thermostat and immersed four times a day for 90 s each time, and stored in artificial saliva for the rest of the time. The treated solution and artificial saliva were collected and replaced daily. After 14 d, the surface hardness of the enamel block was measured with a microhardness meter, 10 mL of the treatment solution was used to measure the pH of each experimental solution using a pH meter, the phosphorus concentration of the treatment solution was measured using the phosphomolybdic acid method, the calcium concentration in the treatment solution was measured using the o-cresyl peptide complexed copper method, the scanning electron microscope was used to observe the surface structure of the enamel block, and the confocal laser scanning microscope was used to observe the depth of enamel block demineralization. Results showed that the deionized water group, cola group and sea buckthorn juice group all showed different degrees of changes on the surface of enamel mass after treatment, the deionized water group observed that the tooth surface changed to chalky color, the cola group and sea buckthorn juice group lost the surface of the sample and saw pigmentation. Deionized water, cola, and Xinjiang sea buckthorn juice all caused a decrease in enamel surface microhardness values, and the differences were statistically significant (P<0.05). The concentration of phosphorus dissolved was from the cola group (15.487±1.168)×10-2 mmol/L, Xinjiang sea buckthorn juice group (8.891±1.423)×10-2 mmol/L and deionized water group (2.191±0.469)×10-2 mmol/L, in descending order, and calcium dissolution concentrations from large to small were Xinjiang sea buckthorn juice group (69.422±1.543)×10-3 mg/mL, cola group (9.744±1.117)×10-3 mg/mL and deionized water group (4.289±0.79)×10-3 mg/mL, and the differences were statistically significant (P< 0.05). Scanning electron microscopy showed that no obvious depressions were found on the surface structure of the samples in the deionized water group, with only sporadic small depressions; the enamel surface in the cola group showed denser small depressions, with some areas connected into sheets; the enamel surface in the Xinjiang sea buckthorn juice group showed a honeycomb structure, with surface depressions connected into sheets. Laser confocal observation showed that the penetration depth in the Xinjiang sea buckthorn juice group was continuous and uniform with the largest A, TF, and AF values; the fluorescent dye in the cola group was discontinuous; and only slight fluorescent dye penetration was seen in the deionized water group. The cola group, the deionized water group and the Xinjiang sea buckthorn juice group all caused enamel surface demineralization, and the degree of demineralization was fast to slow and continuous; the Xinjiang sea buckthorn juice caused the most severe enamel surface demineralization, and the depth of demineralization was stronger than that of cola and deionized water groups.
CHEN Yue
,
WU Zeyu
,
WANG Jing
,
LUO Tiantian
,
SUN Xinxin
,
LIU Yishan
. Assessment of enamel demineralization after exposure to Xinjiang sea buckthorn juice versus cola: An in vitro study[J]. Food and Fermentation Industries, 2022
, 48(16)
: 245
-250
.
DOI: 10.13995/j.cnki.11-1802/ts.030408
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