Bacillus naganoesis pullulanase (PulA) is a starch debranching enzyme that has achieved large-scale production in China.However, its enzymatic properties (especially saccharification pH) are inconsistency with that of glucoamylase in starch saccharification, which effects on the conversion rate of starch to glucose.The characteristics of an acidic mutant, LoPulA, from PulA, and its application value in the production of glucose from starch were studied.The results showed that the maximal activity of LoPulA was at pH 4.5 and 60 ℃ and kept 96.40% of the maximal activity at pH 4.3, which was greatly consistent with the optimal saccharification pH with glucoamylase.In starch saccharification experiments, the DX value of the sugar solution could reach 96.66% with the synergistic hydrolysis of LoPulA and glucoamylase, which was 1.40% higher than that of PulA (wild type) and glucoamylase, thereby indicating LoPulA of great application potential in high glucose syrup preparation from starch.
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