Food and Fermentation Industries >

2022 , Vol. 48 >Issue 17: 175 - 182

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029526

Optimization and preparation of high Fischer ratio peptides from Antarctic krill and its effects on human hepatocellular carcinoma cell line HepG2

  • YAN Shanyao ,
  • CHI Hai ,
  • FENG Guangpeng ,
  • FANG Wenhong ,
  • ZHANG Shuping ,
  • HUANG Yanqing
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  • 1(School of Materials and Chemistry, University of Shanghai for Science and Technology, Shanghai 200093, China)
    2(Key Laboratory of East China Sea and Oceanic Fishery Resource Exploitation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, China Fisheries Research Institute, Shanghai 200090, China)

Received date: 2021-09-27

  Revised date: 2021-12-14

  Online published: 2022-10-01

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Abstract

Firstly, the enzymatic hydrolysis conditions were optimized by using single-factor (material-to-liquid ratio, amount of enzyme, temperature, time) and orthogonal test from defatted Antarctic krill powder in this work. Antarctic krill peptides with high-Fischer ratio (HF-AKP) were prepared by ultrafiltration and followed by activated carbon adsorption. Then the CCK-8 method was used to observe the effect of HF-AKP on the growth of human liver cancer cell line HepG2. Finally, the cell cycle distribution and apoptosis-related protein expression of HepG2 were detected by flow cytometry and ELISA methods, which made efforts to explore the effects of HF-AKP on HepG2 cells. The results revealed that enzymatic hydrolysis conditions of alkaline protease were as follows: the material-to-liquid ratio was 1:17.5 (g:mL) and the addition amount of enzyme was 5.0% for enzymatic hydrolysis at 55℃ for 6 h. The flavor protease optimal conditions were as follows: the addition amount of enzyme was 4.0% and enzymatic hydrolyzed for 3 h. After the second round hydrolysis, the degree of hydrolysis increased up to(17.4±0.2)%. Especially, after activated carbon adsorption, the Fischer ratio of defatted Antarctic krill peptides increased from (2.4±0.2) to (87.2±0.7) compared to AKP. HF-AKP had an inhibitory effect on the cell proliferation of the HepG2, which was positively dependent on dose- and time- manner. It also could block HepG2 cells in the G0/G1 phase. In addition, the mechanism of apoptosis may be related to the increase in Bax/Bcl-2 protein expression ratio and the decrease in NF-κB protein expression, so HF-AKP may have certain anti-tumor effects.

Key words： defatted Antarctic krill powder; double enzymatic hydrolysis; high Fischer ratio peptides; HepG2 cells

Cite this article

YAN Shanyao , CHI Hai , FENG Guangpeng , FANG Wenhong , ZHANG Shuping , HUANG Yanqing . Optimization and preparation of high Fischer ratio peptides from Antarctic krill and its effects on human hepatocellular carcinoma cell line HepG2[J]. Food and Fermentation Industries, 2022 , 48(17) : 175 -182 . DOI: 10.13995/j.cnki.11-1802/ts.029526

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