Affecting factors of soybean protein/egg white protein composite gel performance

  • YANG Juan ,
  • LUO Weiqian ,
  • HE Manyuan
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  • 1(College of Food Science and Engineering, Lingnan Normal University, Zhanjiang 524048, China)
    2(School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China)

Received date: 2022-04-16

  Revised date: 2022-06-07

  Online published: 2022-10-17

Abstract

Egg white protein can be used as the donor of free sulfhydryl groups during the formation of soybean protein heat-induced gel, which can endow the gel with good texture. The gelling properties, water holding capacity and microcosmic properties of soybean protein/egg white protein composite gel were investigated using different factors (reducing agent, ionic strength, heat treatment and polysaccharide). The optimum conditions for inducing disulfide bonds to form composite gel network structure were revealed. The results showed that the addition of egg white protein could increase the disulfide bond content of soybean protein gel, and significantly improve its hardness and water holding capacity. Low concentrations of NaCl, NaHSO3 and gellan gum were helpful to the formation of more disulfide bonds, better texture strength and water holding capacity. Moreover, with the increase of heat treatment time, the protein had more opportunities and sufficient time to open the original disulfide bonds and form more disulfide bonds, and the strength and water holding capacity of the gel formed by the composite system were also improved. Therefore, multi-condition composite gel with NaCl, NaHSO3 and gellan gum had the best gel strength and water holding capacity.

Cite this article

YANG Juan , LUO Weiqian , HE Manyuan . Affecting factors of soybean protein/egg white protein composite gel performance[J]. Food and Fermentation Industries, 2022 , 48(18) : 128 -133 . DOI: 10.13995/j.cnki.11-1802/ts.031999

References

[1] WANG C H, DAMODARAN S.Thermal gelation of globular proteins:Influence of protein conformation on gel strength[J].Journal of Agricultural & Food Chemistry, 1991, 39(3):433-438.
[2] 杨娟, 罗玮倩, 何曼源.大豆蛋白/蛋清蛋白复合凝胶特性的研究[J].食品与发酵工业, 2021, 47(6):134-138.
YANG J, LUO W Q, HE M Y.Study on the characteristics of soy protein/egg white protein composite gelatin[J].Food and Fermentation Industries, 2021, 47(6):134-138.
[3] HATAHET F, BOYD D, BECKWITH J.Disulfide bond formation in prokaryotes:History, diversity and design[J].Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2014, 1 844(8):1 402-1 414.
[4] WANG K Q, LUO S Z, ZHONG X Y, et al.Changes in chemical interactions and protein conformation during heat-induced wheat gluten gel formation[J].Food Chemistry, 2017, 214:393-399.
[5] ZOU L F, FU Y, SHEN K, et al.Sulfur–sulfur bond dissociation enthalpies:A high-level ab initio study[J].Journal of Molecular Structure:THEOCHEM, 2007, 807(1-3):87-92.
[6] 黄群, 杨万根, 金永国, 等.鸭蛋清蛋白凝胶质构特性影响因素研究[J].食品科学, 2014, 35(15):68-71.
HUANG Q, YANG W G, JIN Y G,et al.Factors influencing texture properties of duck egg white protein gel[J].Food Science, 2014, 35(15):68-71.
[7] LI J H, ZHANG M Q, CHANG C H, et al.Molecular forces and gelling properties of heat-set whole chicken egg protein gel as affected by NaCl or pH[J].Food Chemistry, 2018, 261:36-41.
[8] TOMCZYN′SKA-MLEKO M, NISHINARI K, HANDA A.Ca2+-induced eggwhite isolate gels with various microstructure[J].Food Science and Technology Research, 2014, 20(6):1 207-1 212.
[9] WANG W J, SHEN M Y, LIU S C, et al.Gel properties and interactions of Mesona blumes polysaccharide-soy protein isolates mixed gel:The effect of salt addition[J].Carbohydrate Polymers, 2018, 192:193-201.
[10] GUO J, YANG X Q, HE X T, et al.Limited aggregation behavior of β-conglycinin and its terminating effect on glycinin aggregation during heating at pH 7.0[J].Journal of Agricultural & Food Chemistry, 2012, 60(14):3 782-3 791.
[11] VAN DER PLANCKEN I, VAN LOEY A, HENDRICKX M E G.Changes in sulfhydryl content of egg white proteins due to heat and pressure treatment[J].Journal of Agricultural & Food Chemistry, 2005, 53(14):5 726-5 733.
[12] LAMBRECHT M A, ROMBOUTS I, DE KETELAERE B, et al.Prediction of heat-induced polymerization of different globular food proteins in mixtures with wheat gluten[J].Food Chemistry, 2017, 221:1 158-1 167.
[13] 许怀远, 任向妍, 王垚.结冷胶产品凝胶特性的影响因素研究[J].食品科技, 2009, 34(11):263-268.
XU H Y, REN X Y, WANG Y.Study on affecting factors for gel properties of gellan gum[J].Food Science and Technology, 2009, 34(11):263-268.
[14] HOGG P J.Disulfide bonds as switches for protein function[J].Trends in Biochemical Sciences, 2003, 28(4):210-214.
[15] VISSCHERS R W, DE JONGH H H J.Disulphide bond formation in food protein aggregation and gelation[J].Biotechnology Advances, 2005, 23(1):75-80.
[16] 阮奇珺. 大豆蛋白热诱导二硫键连接物的形成及巯基变化亚基水平研究[D].无锡:江南大学, 2015.
RUAN Q J.Subunit level study on heat induced disulfide linked products and sulfhydryl change of soy protein[D].Wuxi:Jiangnan University, 2015.
[17] HANDA A, TAKAHASHI K, KURODA N, et al.Heat-induced egg white gels as affected pH[J].Journal of Food Science, 1998, 63(3):403-407.
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