Food and Fermentation Industries >

2022 , Vol. 48 >Issue 18: 162 - 167

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030148

Physicochemical properties of Morchella sextelata mycelium under salt stress

  • YANG Tong ,
  • SUN Jing ,
  • HAO Chen ,
  • WANG Jianrui ,
  • LIU Yu
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  • (School of Agriculture, Ludong University, Yantai 264000, China)

Received date: 2021-11-19

  Revised date: 2021-12-27

  Online published: 2022-10-17

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Abstract

Taking Morchella sextelata M3 as the research object, the effect of salt stress on mycelium was investigated. Water extraction and alcohol precipitation method, ultrasonic assisted extraction method, DPPH reduction method, H2O2/Fe system method and potassium ferricyanide reduction method were used to determine the changes of mycelium microstructure, intracellular and extracellular polysaccharide content, DPPH free radical, hydroxyl radical scavenging rate and reduction ability under salt stress at different times. The results showed that the mycelium diameter decreased slowly with the increase of salt stress time. The polysaccharide content of salt-containing mycelia was higher than that of normal mycelia at 3 h, which was 12.74 mg/g, and then the content was lower than that of normal mycelia. The content of extracellular polysaccharides under salt stress was lower than that of normal content, but they both increased first and then decreased. The highest content was 0.14 mg/mL and 0.29 mg/mL at 12 h, respectively. Salt stress promoted the scavenging rate of DPPH free radical and hydroxyl free radical, especially DPPH free radical scavenging capacity, intracellular polysaccharide clearance capacity increased by 3.47 times, and extracellular polysaccharide increased by 6.72 times. The effect of reducing power on intracellular polysaccharide was greater than that on extracellular polysaccharide. In summary, this study provides a theoretical basis for exploring the physiological activity of Morchella sextelata under salt stress.

Key words： salt stress; microstructure; intracellular polysaccharide; extracellular polysaccharide; antioxidant activity

Cite this article

YANG Tong , SUN Jing , HAO Chen , WANG Jianrui , LIU Yu . Physicochemical properties of Morchella sextelata mycelium under salt stress[J]. Food and Fermentation Industries, 2022 , 48(18) : 162 -167 . DOI: 10.13995/j.cnki.11-1802/ts.030148

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