Food and Fermentation Industries >

2022 , Vol. 48 >Issue 18: 244 - 248

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029579

Fast determination of gibberellin(GA3)residues in edible amaranth and spinach by liquid chromatography-tandem mass spectrometry

  • YE Qian ,
  • TANG Xuemei ,
  • GAO Yuwen ,
  • HUANG Jianxiang
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  • 1(Institute of Quality Standard and Monitoring Technology for Agro-products of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China)
    2(Laboratory of Quality and Safety Risk Assessment of Agro-product (Guangzhou), Ministry of Agriculture and Rural, Guangzhou 510640, China)
    3(Guangdong Agricultural Monitoring Technology Co.Ltd.,Guangzhou 510640, China)

Received date: 2021-10-09

  Revised date: 2021-11-24

  Online published: 2022-10-17

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Abstract

An analytical method was developed for determination of gibberellin (GA3) residues in edible amaranth and spinach. Sample preparation method was based on QuEChERS. GA3 was extracted by methanol containing 1% (volume ratio) acetic acid and detected with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The optimization of extraction solvent, extraction time, adsorbent, type of salt and dilution factor was studied. The results showed that the linear ranges of GA3 were between 0.001and 1.0 mg/L. The limit of detection was 0.001 mg/kg. Furthermore, under the addition of 0.010, 0.10 and 0.50 mg/kg, the recovery of GA3 in edible amaranth and spinach reached 82%-91%, and the relative standard deviation was 2%-6%. The limit of quantification was 0.010 mg/kg which was the lowest spiked concentration. Then, the risk of GA3 in 131 amaranth and 331 spinach samples were monitored by this method. The results showed that the maximum residue of GA3 was 0.16 mg/kg. According to the maximum residue limit of GA3 in edible amaranth in vegetables in Japan positive list, no samples exceeded the standard. The method is fast, easy and accurate, which can meet the requirements for the determination of GA3 residues in edible amaranth and spinach.

Key words： gibberellin; QuEChERS; liquid chromatography-tandem mass spectrometry; edible amaranth; spinach; residue

Cite this article

YE Qian , TANG Xuemei , GAO Yuwen , HUANG Jianxiang . Fast determination of gibberellin(GA3)residues in edible amaranth and spinach by liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2022 , 48(18) : 244 -248 . DOI: 10.13995/j.cnki.11-1802/ts.029579

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