In order to help consumers understand the quality and safety of goat milk powder sold in China and abroad, and promote enterprises to improve the quality of goat milk powder, DNA extraction and quality testing were carried out on 38 brands of goat milk powder sold in China and abroad, and the bovine-derived components in goat milk powder were qualitatively and quantitatively detected by ordinary PCR and quantitative real-time PCR. The results showed that the OD value of DNA in commercial goat milk powder was 1.3-1.5 with a concentration of 200-500 ng/μL. The purity and concentration of DNA in pure goat milk powder were significantly higher than that of formula goat milk powder (P＜0.05). Moreover, DNA had different degrees of degradation, however the PCR amplification could meet the requirements of subsequent adulteration detection test. Both ordinary PCR and quantitative real-time PCR could detect at least 0.1% of bovine-derived components. The quantitative real-time PCR could quantify 0.1%-50% bovine-derived components in the samples. The qualitative adulteration test of 38 kinds of commercially available goat milk powder found that 4 kinds of goat milk powder could be detected bovine-derived ingredients, which were not consistent with the product label. Further quantitative results found that the content of bovine-derived ingredients in 3 kinds of goat milk powder was about 1%, and the content of bovine-derived ingredients in a kind of goat milk powder was higher than 50%. Therefore, PCR-based methods for the detection of adulterated bovine-derived components in goat milk powder, which could provide a reliable basis for the verification of goat milk powder label claims and industrial quality control, and it could also ensure the purity of commercially available goat milk powder products.
ZHANG Xueru
,
FU Shangchen
,
ZHENG Weimin
,
LI Ling
,
LIU Yongfeng
. Quality evaluation of DNA in commercial goat milk powder and the detection of adulterated bovine-derived components[J]. Food and Fermentation Industries, 2022
, 48(18)
: 249
-255
.
DOI: 10.13995/j.cnki.11-1802/ts.029467
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