In order to study the heterogeneity of tolerance to low temperature (4 °C) of different Staphylococcus aureus strains, as well as its relationship between the cell membrane integrity and related gene expression. The low temperature tolerances of four isolated S. aureus strains were compared by plating counting prior to viable cell counting. Results showed that after four weeks of low temperature treatment, the BB-1 strain could form small colony variants (SCVs) compared to other strains, which also exhibited the strongest tolerance to low temperature and the survival rate reached 75.50 %. Comparatively, the BH-25 strain with the weakest tolerance was unable to form SCVs and the survival rate was only 45.68 %. Subsequently, the leakage of intracellular contents, the conductivity of the bacterial suspension, and the crystal violet staining analysis were performed to study the effect of low temperature on the cell membrane permeability. The results indicated that the integrity of the BB-1 cell membrane is stronger than BH-25. Thereafter, the quantitative real-time PCR was employed to analyze the regulation patterns of the stress tolerance related genes (rsbV, rsbW and sigB) and the cell membrane fatty acid biosynthesis related genes (fabD, fabF, fabG, fabH and fabI). The result indicated that the transcriptional levels of the tested genes were significantly higher in BB-1 strain than those in BH-25 (P<0.05). The tested four S. aureus strains exhibited significant difference in the tolerance to low temperature, BB-1 that can form SCVs has a stronger survival capability at low temperature, while the cell membrane is more intact to inhibit the leakage of cellular content. Moreover, the expression levels of rsbV-rsbW/sigB system genes and fab series genes that regulate cell membrane fatty acid biosynthesis in SCVs cells were significantly higher than the strain that could not form SCVs. The present study offers a newly basis for clarifying the mechanism of S. aureus low temperature tolerance, as well as for guiding the development of safety control technology for cold-chain food.
GUAN Peng
,
DONG Zijie
,
HE Shujia
,
LIU Yueyang
,
LEI Mengmeng
,
HUANG Zhongmin
,
AI Zhilu
,
SUO Biao
. Effects of low temperature on cell membrane integrity and related gene expression of Staphylococcus aureus strains with different stress tolerance[J]. Food and Fermentation Industries, 2022
, 48(19)
: 16
-22
.
DOI: 10.13995/j.cnki.11-1802/ts.030623
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