Phenylketonuria is an amino acid metabolic disorder caused by the deficiency of phenylalanine hydroxylase, which leads to the over-accumulation of phenylalanine in body. The phenylalanine-restricted low-protein diet plays a significant role of nutrition supply to phenylketonuria patients. The aim of this study was to screen the probiotics with phenylalanine-degrading activity and evaluate the potential application of fermented milk production. The results showed that Lactobacillus plantarum YZX21 and Bifidobacterium animalis F1-7 exhibited high phenylalanine degradation ability. The degradation rates were 12.43% and 10.09% respectively. Moreover, L. plantarum YZX21 and B. animalis F1-7 had good gastrointestinal tolerance, self-aggregation and adhesion ability. Compared with B. animalis F1-7, L. plantarum YZX21 showed better fermentation properties and could be used as an auxiliary starter strain to produce fermented milk. As well as compared with the commercially fermented milk, the content of phenylalanine in fermented milk inoculated with L. plantarum YZX21 was decreased by 13.10%. In addition, the sensory characteristics of the fermented milk with L. plantarum YZX21was better than that of commercially fermented milk. These findings indicated that L. plantarum YZX21 had high phenylalanine degradation ability, excellent fermentation and probiotic properties, which laid the foundation for the development of foods for special medical purpose to phenylketonuria patients.
MA Xinying
,
JI Yuanyuan
,
LIU Ao
,
ZHOU Wei
,
YIN Boxing
,
LIU Tongjie
,
GONG Pimin
,
ZHANG Lanwei
,
YI Huaxi
. Screening of phenylalanine-degrading probiotics and its application in yogurt fermentation[J]. Food and Fermentation Industries, 2022
, 48(19)
: 204
-209
.
DOI: 10.13995/j.cnki.11-1802/ts.031031
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