As a renewable agroforestry resource, corn stover is rich in lignocellulose, which can produce xylose, glucose, arabinose and other sugars after being hydrolyzed by acid. These sugars can be used as a carbon source for the fermentation of Bacillus subtilis to produce acetoin, which has important economic and social benefits in the development of new energy and environmental protection. The effects of hydrolysis acid concentration, hydrolysis temperature and hydrolysis time on the yield of sugars in corn stover hydrolysate were investigated by response surface design, and the optimal pretreatment conditions were 120 ℃, sulfuric acid concentration of 1.0% and hydrolysis time of 1 h, under which the damage to the fiber structure of corn stover was reduced while obtaining more sugars. The yield of acetoin reached the maximum of 10.4 g/L until the 5th day by the fermentation of B. subtilis in the 5 L automatic fermenter with the mixture of hydrolyzate of corn stover and LB medium. And the hydrolyzate residue could be used in papermaking, synergistically improving the comprehensive utilization of corn stover.
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