Jasmonic acid is a plant endogenous hormone that plays an important role in the process of plant resistance to biotic and abiotic stress. The filamentous fungus Lasiodiplodia sp. can secrete jasmonic acid under static culture, while the mechanism of jasmonic acid synthesis is still unclear. In this study, Lasiodiplodia iranensis CCTCC M2017188 was selected as the research object, and L. iranensis was cultured under static and shaking conditions to investigate the influence mechanism of culture environment on jasmonic acid synthesis. The fermentation parameters under two conditions were investigated, and metabolic intermediates were collected for metabolomics analysis.There were significant differences in the metabolic characteristics of L. iranensis under two conditions. The biomass under shaking culture was higher, the substrate consumption rate was faster, while the yield of jasmonic acid was lower, compared with static culture. In addition, the pH fluctuatuion and the morphology of mycelium were different from those in static culture. Through non-targeted metabolomics analysis, a total of 77 different metabolites were identified, 26 of which were enriched in the KEGG metabolic pathway. Comparing static and shaking culture, the metabolic pathway that contains the most down-regulated metabolites was amino acid metabolism, among which dulcitol, S-adenosyl-L-homocysteine, methylmalonic acid, adenine, guanosine and L-tryptophan were down-regulated. And another significantly down-regulated metabolic pathway was nucleotide metabolism. While there are only two different metabolites in lipid metabolism, among which jasmonic acid is significantly up-regulated. Under shaking condition, the nutrients ingested by fungi flew more to amino acid metabolism and nucleotide metabolism, while the flux to jasmonic acid synthesis was relatively reduced. In addition, jasmonic acid synthesis was competitively inhibited by traumatic acid and 3-hexenol metabolic pathways. This explained the reason for the low yield of jasmonic acid under shaking condition to some extent, and is meaningful for understanding the mechanism of jasmonic acid synthesis by fungi.
沈子强
,
郑璞
,
李睿英
,
吴丹
,
陈鹏程
. Insight into the metabolic differences of jasmonic acid-producing Lasiodiplodia iranensis under static and shaking conditions[J]. Food and Fermentation Industries, 2022
, 48(20)
: 91
-97
.
DOI: 10.13995/j.cnki.11-1802/ts.030644
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