A method for the determination of cyromazine residues in 14 kinds of vegetables including garlic chives by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQMS) was established. The samples were extracted with acetonitrile, and then 5 mL supernatant liquid was evaporated with a nitrogen stream and dissolved in 1 mL acetonitrile. The dissolving solution was separated with a hydrophilic chromatography column by isocratic elution using 5 mmol/L ammonium acetate solution containing 0.1% formic acid with acetonitrile as mobile phases. Then, it was detected by electrospray ion source positive ion detection mode and multiple reaction monitoring modes, and quantified by the matrix-matched external standard calibration curves. Cyromazine had a good linear relationship in the concentration range of 0.5-100 μg/L with good correlation coefficients (r>0.99). The average recoveries ranged from 80% to 113% for cyromazine at 3 spiked levels of 2.0, 4.0, and 20.0 μg/kg. The relative standard deviations (RSD, n=3) were in the range of 0.56%-7.21%. The limits of detection were 0.5 μg/kg (S/N=3) and the limits of quantification detection were 2.0 μg/kg(S/N=10).
俞所银
,
闫晴
,
梁佳
. Determination of cyromazine residues in 14 kinds of vegetables by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQMS)[J]. Food and Fermentation Industries, 2022
, 48(20)
: 231
-237
.
DOI: 10.13995/j.cnki.11-1802/ts.029826
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