Food and Fermentation Industries >

2022 , Vol. 48 >Issue 21: 146 - 151

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030728

Preparation and characteristics of antibacterial nanofiber membrane containing bacteriocin CAMT2

  • LI Qibin ,
  • ZHANG Xuemei ,
  • LIU Ying ,
  • FANG Zhijia ,
  • XU Chunhou
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  • 1 (College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang 524088, China)
    2(College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China)

Received date: 2022-01-13

  Revised date: 2022-03-01

  Online published: 2022-12-02

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Abstract

The bacteriocin CAMT2 from Bacillus amyloliquefaciens ZJHD3-06 was used as the antimicrobial active ingredient and polyvinyl alcohol (PVA) was as the encapsulation material. The antibacterial CAMT2—PVA nanofiber membranes were prepared by electrostatic spinning technique, and the inhibition activity, release effect, structure and inhibition effect of the produced nanofiber membranes against Listeria monocytogenes in common aquatic matrices at low temperature were evaluated. The results showed that the CAMT2—PVA nanofibers membranes with a drug loading of 80 mg/mL retained good antibacterial activity, with a quick release phase of 0.5-2 d, a slow-release phase on the second day and a stabilization phase after the seventh day. Scanning electron microscope showed that the surface of the fibers was smooth and flat, without irregularities such as beads, and the diameter of the fibers was 720 nm; the results of Fourier transform infrared showed that the bacteriocin CAMT2 was effectively encapsulated by PVA during the electrospinning process; the inhibition effect of CAMT2—PVA nanofibers membrane was significant in three typical aquatic substrates. This suggests that CAMT2—PVA has good potential applications.

Key words： bacteriocin; electrospinning; nanofiber; antibacterial; structure

Cite this article

LI Qibin , ZHANG Xuemei , LIU Ying , FANG Zhijia , XU Chunhou . Preparation and characteristics of antibacterial nanofiber membrane containing bacteriocin CAMT2[J]. Food and Fermentation Industries, 2022 , 48(21) : 146 -151 . DOI: 10.13995/j.cnki.11-1802/ts.030728

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