Analysis of volatile flavor profile and characteristics of rosy vinegar based on HS-SPME-GC-MS profiles

  • ZHANG Linxiang ,
  • ZHANG Lei ,
  • QIN Zihan ,
  • CHEN Jiahui ,
  • XING Limin ,
  • ZHU Junli
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  • 1(School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China)
    2(Zhejiang Laohenghe Food Co.Ltd., Huzhou 313213, China)

Received date: 2022-01-12

  Revised date: 2022-02-25

  Online published: 2022-12-02

Abstract

In this study, the physical and chemical indicators, sensory characteristics and flavor profile of six kinds of rosy vinegar from various origins were analyzed. The results showed that the pH value, acidity and amino acid nitrogen content of six rosy vinegars had no significant differences, of while the M5 reducing sugar content of rosy vinegar samples was significantly higher than other groups. Sensory analysis found that attributes of rosy vinegars differed significantly, including fruit aroma, bran aroma, caramel aroma, and aroma comprehensive complexity, especially bran aroma. HS-SPME-GC-MS analysis showed that total 76 volatile compounds were identified in rosy vinegar, mainly containing esters, acids, aldehydes and ketones, and rosy vinegar produced in Zhejiang have more flavor compounds. Seven volatile compounds with odor activity value (OAV) greater than 1 were calculated based on threshold, which were isoamyl aldehydes, benzaldehyde, ethyl acetate, isoamyl acetate, 3-acetyl-2-butanone, 2,3-butanedione, 2-acetylfuran. In addition, ethyl acetate, 3-acetyl-2-butanone and 2,3-butanedione were identified as the characteristic flavor components of Zhejiang rosy vinegar. Orthogonal partial least squares discriminant analysis (OPLS-DA) and variable importance in projection (VIP) indicated that 3-methyl butanal, ethyl acetate and isoamyl acetate might be associated with the difference in aroma of rosy vinegar.

Cite this article

ZHANG Linxiang , ZHANG Lei , QIN Zihan , CHEN Jiahui , XING Limin , ZHU Junli . Analysis of volatile flavor profile and characteristics of rosy vinegar based on HS-SPME-GC-MS profiles[J]. Food and Fermentation Industries, 2022 , 48(21) : 269 -275 . DOI: 10.13995/j.cnki.11-1802/ts.031133

References

[1] 陈笑钗, 李婷, 黄炳文, 等.浙江玫瑰醋补料发酵工艺条件的优化[J].中国调味品, 2019, 44(8):152-157.
CHEN X C, LI T, HUANG B W, et al.Optimization of fed-batch fermentation process conditions for rose vinegar in Zhejiang Province[J].China Condiment, 2019, 44(8):152-157.
[2] 方冠宇. 浙江玫瑰醋搅拌工艺及发酵过程中微生物与风味物质相关性研究[D].杭州:浙江工商大学, 2019.
FANG G Y.Research on the stirring technology and the correlation between microorganism and flavor substance in Zhejiang rosy vinegar fermentation process[D].Hangzhou:Zhejiang Gongshang Unniversity, 2019.
[3] WANG Z M, LU Z M, YU T J, et al.Batch-to-batch uniformity of bacterial community succession and flavor formation in the fermentation of Zhenjiang aromatic vinegar[J].Food Microbiology, 2015, 50:64-69.
[4] ZHU Y P, ZHANG F F, ZHANG C N, et al.Dynamic microbial succession of Shanxi aged vinegar and its correlation with flavor metabolites during different stages of acetic acid fermentation[J].Scientific Reports, 2018, 8:8612.
[5] 周利南, 林森, 蒋予箭, 等.保温后熟及陈酿条件与玫瑰醋色泽形成的相关性研究[J].中国酿造, 2009, 28(6):38-41.
ZHOU L N, LIN S, JIANG Y J, et al.Influence of heat treatment and storage on color of rosy vinegar[J].China Brewing, 2009, 28(6):38-41.
[6] ZHOU Z L, JIAN D Z, GONG M, et al.Characterization of the key aroma compounds in aged Zhenjiang aromatic vinegar by gas chromatography-olfactometry-mass spectrometry, quantitative measurements, aroma recombination and omission experiments[J].Food Research International, 2020, 136:109434
[7] 孙优兰, 黄永光, 唐东亚, 等.基于香气活性值及感官属性对金银花尾酒酿造风味食醋特征的分析[J].食品与发酵工业, 2019, 45(15):233-242.
SUN Y L, HUANG Y G, TANG D Y, et al.Analysis of flavor characteristics of vinegar brewed by honeysuckle tail liquor based on odor activity value and sensory attributes[J].Food and Fermentation Industries, 2019, 45(15):233-242.
[8] 孙宗保. 镇江香醋挥发性成分分析及醋龄的识别研究[D].镇江:江苏大学, 2014.
SUN Z B.Analysis of volatile components of Zhenjiang balsamic vinegar and identification study of vinegar age[D].Zhenjiang:Jiangsu University, 2014.
[9] 里奥·范海默特.化合物香味阈值汇编[M].北京:科学出版社, 2015.
VAN HEMERT L.Compilation of Compound Fragrance Thresholds[M].Beijing:Science Press, 2015.
[10] WU J J, MA Y K, ZHANG F F, et al.Biodiversity of yeasts, lactic acid bacteria and acetic acid bacteria in the fermentation of “Shanxi aged vinegar”, a traditional Chinese vinegar[J].Food Microbiology, 2012, 30(1):289-297.
[11] FANG G Y, WU G Z, JIANG Y J.Production of Zhejiang rosy vinegar by pneumatic stirring and its effect on product flavor[J].Food Science, 2019, 40(18):161-172.
[12] 郑宇, 程程, 刘静, 等.中国传统固态发酵食醋主要特征风味物质组成分析[J].中国食品学报, 2020, 20(8):237-247.
ZHENG Y, CHENG C, LIU J, et al.Analysis of main characteristic flavor components in Chinese traditional solid-state fermented vinegars[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(8):237-247.
[13] 穆晓静, 方冠宇, 蒋予箭.基于多元统计方法分析陈酿条件对浙江玫瑰醋香气成分的影响[J].食品科学, 2020, 41(14):262-270.
MU X J, FANG G Y, JIANG Y J.Effect of aging conditions on volatile flavor compounds of Zhejiang rosy vinegar evaluated by multivariate statistical analysis[J].Food Science, 2020, 41(14):262-270.
[14] 孔祥伟, 周志磊, 郑福平, 等.中国酿造食醋风味轮的构建及应用[J].食品与生物技术学报, 2020, 39(2):74-80.
KONG X W, ZHOU Z L, ZHENG F P, et al.Construction of flavor wheel for the brewing vinegar in China and its applications[J].Journal of Food Science and Biotechnology, 2020, 39(2):74-80.
[15] 杜大钊, 黄静, 王瑞, 等.四川麸醋及其陈酿过程中挥发性风味物质解析[J].食品与发酵科技, 2020, 56(5):1-6.
DU D Z, HUANG J, WANG R, et al.Analysis of volatile flavor substances in Sichuan bran vinegar and its aging process[J].Food and Fermentation Sciences & Technology, 2020, 56(5):1-6.
[16] DONG D, ZHENG W, JIAO L, et al.Chinese vinegar classification via volatiles using long-optical-path infrared spectroscopy and chemometrics[J].Food Chemistry, 2016, 194:95-100.
[17] 李江涌, 王如福, 焦玉双.山西老陈醋熏醅前后香气成分的变化分析[J].中国酿造, 2016, 35(9):47-50.
LI J Y, WANG R F, JIAO Y S.Changes of aroma components in Shanxi aged vinegar during fumigation process[J].China Brewing, 2016, 35(9):47-50.
[18] 孙宗保, 赵杰文, 邹小波, 等.镇江香醋特征香气成分加工过程中的变化和形成机理分析[J].中国食品学报, 2010, 10(3):120-127.
SUN Z B, ZHAO J W, ZOU X B, et al.Changes of characteristic aroma component in Zhenjiang fragrance vinegar during processing and analysis of their formation mechanism[J].Journal of Chinese Institute of Food Science and Technology, 2010, 10(3):120-127.
[19] ETSCHMANN M M W, BLUEMKE W, SELL D, et al.Biotechnological production of 2-phenylethanol[J].Applied Microbiology and Biotechnology, 2002, 59(1):1-8.
[20] XU S Z, MA Z W, CHEN Y, et al.Characterization of the flavor and nutritional value of coconut water vinegar based on metabolomics[J].Food Chemistry, 2022, 369:130872.
[21] 孙宗保, 樊毅萍, 冀鹏.基于SPME-GC-MS-O的不同品牌镇江香醋香气的比较研究[J].中国调味品, 2015, 40(9):26-29.
SUN Z B, FAN Y P, JI P.Comparative study on the flavor compounds of three brands of Zhenjiang aromatic vinegar analyzed by SPME-GC-MS-O[J].China Condiment, 2015, 40(9):26-29.
[22] 王瑞, 彭晓光, 黄登宇.食醋中川芎嗪成分研究进展[J].中国调味品, 2015, 40(6):137-140.
WANG R, PENG X G, HUANG D Y.Research progress of tetramethylpyrazine in vinegar[J].China Condiment, 2015, 40(6):137-140.
[23] 杨霁虹, 周汉琛, 刘亚芹, 等.基于HS-SPME-GC-MS和OAV值分析黄山地区不同茶树品种红茶香气的差异[J/OL].食品科学, 1-13.http://kns.cnki.net/kcms/detail/11.2206.TS.20211015.2013.006.html.
YANG J H, ZHOU H S, LIU Y Q, et al.Analysis of aroma components of black teas processed by different tea cultivars in Huangshan using HS-SPME-GC-MS and OAV[J/OL].Food Science, 1-13.http://kns.cnki.net/kcms/detail/11.2206.TS.20211015.2013.006.html.
[24] 邝格灵, 李树, 宁甜甜, 等.基于GC-MS指纹图谱和多元统计学区分四川保宁醋和山西老陈醋的挥发性差异代谢产物[J].食品科学, 2020, 41(16):227-232.
KUANG G L, LI S, NING T T, et al.Differential volatile metabolites between Sichuan baoning vinegar and Shanxi aged vinegar determined by GC-MS fingerprint and multivariate statistics[J].Food Science, 2020, 41(16):227-232.
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