Food and Fermentation Industries >

2022 , Vol. 48 >Issue 22: 54 - 59

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030293

Optimization of production process of Lactobacillus plantarum microencapsules and its properties

  • YAO Jingwei ,
  • ZHU Hanfang ,
  • HUANG Xuecheng ,
  • GAO Xin ,
  • GUO She ,
  • CHEN Xia
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  • (Key Laboratory of Dairy Biotechnology and Engineering of Ministry of Education, Key Laboratory of Dairy Processing of Ministry of Agriculture and Rural Areas, Key Laboratory of Dairy Biotechnology and Engineering of Inner Mongolia Autonomous Region, Inner Mongolia Agricultural University, Hohhot 010018, China)

Received date: 2021-12-01

  Revised date: 2021-12-25

  Online published: 2022-12-20

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Abstract

Accomplishing an adequate level of colonization in intestinal tract is one of the essentials of probiotics to confer beneficial effects to the consumer. However, some strains are unable to effectively exert beneficial effects due to poor tolerance to gastric acid, digestive enzymes, bile acids, and others. Here, we designed a study to establish the microencapsulation technology of Lactobacillus plantarum IMAU10120-1 with sodium alginate as wall material by using the microcapsule granulator B-390. The concentration of sodium alginate, CaCl2, and curing time was optimized. Results unraveled that the optimal preparation conditions for L. plantarum IMAU10120-1 were 1.8% sodium alginate, 3.0% CaCl2, and 40 min curing time. The particle size of the prepared microencapsules was uniform (about 300 μm), and the embedding rate reached to 85.1%. In simulated intestinal liquid (without bovine bile salt), 1.55×1010 CFU/mL cells were obtained after being treated for 60 min, which showed marked enteric solubility. Microencapsulation can effectively increase the tolerance of L.plantarum IMAU10120-1 to simulated gastric conditions.After treatment in simulated gastric juice for 3 h, the survival rate of microcapsules in gastric juice was 85.68%. Additionally, when L. plantarum IMAU10120-1 was transferred into simulated intestinal juice and treated for 8 h, the survival rate was 79.36%. This study may provide certain seminal data to develop microencapsulation tech- nology for Lactobacillus spp.

Key words： Lactobacillus plantarum; microencapsulation; sodium alginate; bedding rate; intestinal solubility

Cite this article

YAO Jingwei , ZHU Hanfang , HUANG Xuecheng , GAO Xin , GUO She , CHEN Xia . Optimization of production process of Lactobacillus plantarum microencapsules and its properties[J]. Food and Fermentation Industries, 2022 , 48(22) : 54 -59 . DOI: 10.13995/j.cnki.11-1802/ts.030293

References

[1] 曹承旭, 郭晶晶, 乌日娜, 等.植物乳杆菌的生理功能和组学研究进展[J].乳业科学与技术, 2018, 41(1):33-39.
CAO C X, GUO J J, WU R N, et al.Advances in understanding the physiological function and omics of Lactobacillus plantarum [J].Journal of Dairy Science and Technology, 2018, 41(1):33-39.
[2] 陈婷, 王玉华, 蔡丹, 等.益生菌微胶囊技术研究进展[J].中国乳品工业, 2016, 44(1):31-37.
CHEN T, WANG Y H, CAI D, et al.Advances in wall materials and methods of probiotic microencapsulation[J].China Dairy Industry, 2016, 44(1):31-37.
[3] 何云侠. 干酪乳杆菌微胶囊的制备及应用[D].西安:陕西科技大学, 2019.
HE Y X.Preparation and application of Lactobacillus casei microcapsules[D].Xi'an:Shaanxi University of Science and Technology, 2019.
[4] MU R J, YUAN Y, WANG L, et al.Microencapsulation of Lactobacillus acidophilus with konjac glucomannan hydrogel[J].Food Hydrocolloids, 2018, 76:42-48.
[5] 宋娇娇, 包秋华, 王亚利, 等.瑞士乳杆菌MG9-2微胶囊的制备工艺优化及其性能分析[J].食品科学, 2017, 38(14):49-57.
SONG J J, BAO Q H, WANG Y L, et al.Optimization of preparation process and properties of microcapsules containing Lactobacillus helveticus MG9-2[J].Food Science, 2017, 38(14):49-57.
[6] 聂福贵. 厚垣普可尼亚菌微胶囊的研制[D].呼和浩特:内蒙古农业大学, 2019.
NIE F G.Preparation and study of microcapsule of Pochonia chlamydosporia[D].Hohhot:Inner Mongolia Agricultural University, 2019.
[7] 李洪波, 李春爽, 张天琪, 等.干酪乳杆菌/乳糖醇双层合生元微胶囊制备及其在橙汁中的应用[J].中国食品学报, 2020, 20(4):93-99.
LI H B, LI C S, ZHANG T Q, et al.Preparation of Lactobacillus casei/lactitol synbiotic microcapsule and its application in orange juice[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(4):93-99.
[8] FRAKOLAKI G, KEKES T, LYMPAKI F, et al.Use of encapsulated Bifidobacterium animalis subsp.lactis through extrusion or emulsification for the production of probiotic yogurt[J].Journal of Food Process Engineering, 2022, 45(7):1-8.
[9] 王欢, 肖军霞, 黄国清, 等.微胶囊造粒仪制备青春双歧杆菌微胶囊[J].食品研究与开发, 2016, 37(21):92-97.
WANG H, XIAO J X, HUANG G Q, et al.Studies on preparation of Bifidobacterium adolescentis microcapsules using encapsulator B-395 Pro[J].Food Research and Development, 2016, 37(21):92-97.
[10] 陈美瑄.母乳中益生乳杆菌和双歧杆菌的初步筛选及特性研究[D].呼和浩特:内蒙古农业大学, 2020.
CHEN M X. Preliminary screening and characterization of probiotic Lactobacillus and Bifidobacteriumisolated from human breast milk[D]. Hohhot:Inner Mongolia Agricultural University, 2020.
[11] TIAN W J, SONG J J, WANG Y L, et al. Effect of different calcium salts and methods for triggering gelation on the characteristics of microencapsulated Lactobacillus plantarum LIP-1[J]. RSC Advances, 2015, 5(90):73 352-73 362.
[12] 张生生, 闫静芳, 陆兆新, 等. 锐孔凝固浴法制备表面活性肽微胶囊[J]. 食品工业科技, 2015, 36(12):221-225;230.
ZHANG S S, YAN J F, LU Z X, et al. The preparation of surfactin microcapsules by the method of piercing-solidifying [J].Science and Technology of Food Industry, 2015, 36(12):221-225;230.
[13] 李作美, 许晓云, 刘琦. 双歧杆菌微胶囊的制备及其理化性能[J]. 食品与发酵工业, 2020, 46(6):155-162.
LI Z M, XU X Y, LIU Q. Preparation and physical and chemical properties of Bifidobacterium microcapsules[J]. Food and Fermentation Industries, 2020, 46(6):155-162.
[14] CHÁVARRI M, MARAÑÓN I, ARES R, et al. Microencapsulation of a probiotic and prebiotic in alginate-chitosan capsules improves survival in simulated gastro-intestinal conditions[J]. International Journal of Food Microbiology, 2010, 142(1-2):185-189.
[15] ZHAO Y P, LIU S, FENG Y Q, et al. Development and optimization of attapulgite clay based microencapsulation for lactic acid bacteria by response surface methodology[J]. International Journal of Food Engineering, 2019, 15(8):21-25.
[16] CHANDRAMOULI V, KAILASAPATHY K, PEIRIS P, et al. An improved method of microencapsulation and its evaluation to protect Lactobacillus spp. in simulated gastric conditions[J].Journal of Microbiological Methods, 2004, 56(1):27-35.
[17] 周莉, 王晓瑞, 平洋, 等. 保加利亚乳杆菌微胶囊的制备及特性研究[J]. 食品工业科技, 2020, 41(11):188-193.
ZHOU L, WANG X R, PING Y, et al. Preparation and characterization of microcapsule Lactobacillus bulgaricus[J]. Science and Technology of Food Industry, 2020, 41(11):188-193.
[18] 马岩, 王艳杰, 陈昱凤. 复合壁材乳酸杆菌微胶囊的制备及其耐贮性和耐受性[J]. 农业工程, 2016, 6(3):54-58.
MA Y, WANG Y J, CHEN Y F.Preparation of compound wall material Lactobacillus microcapsule and its storability and tolerance[J]. Agricultural Engineering, 2016, 6(3):54-58.
[19] 郭宇星, 潘道东, 刘洋, 等.海藻酸钠固定瑞士乳杆菌条件优化[J]. 食品科学, 2012, 33(5):188-192.
GUO Y X, PAN D D, LIU Y, et al. Optimization of conditions for immobilization of Lactobacillus helveticus using sodium alginate[J]. Food Science, 2012, 33(5):188-192.
[20] 王庆卫. 内源乳化法制备双歧杆菌微胶囊的研究[D].无锡:江南大学, 2014.
WANG Q W. Study on the Bifidobacterium-loaded microcapsule prepared by internal emulsification method[D].Wuxi:Jiangnan University, 2014.
[21] 卢晓霆, 许中畅, 王田田, 等. 玉米多孔淀粉-海藻酸钠-壳聚糖-葡萄多酚缓释微胶囊的制备及表征[J]. 中草药, 2016, 47(13):2 252-2 259.
LU X T, XU Z C, WANG T T, et al.Preparation and characterization of sustained release microcapsules of grape polyphenols with porous cornstarch, alginate sodium, and chitosan[J]. Chinese Traditional and Herbal Drugs, 2016, 47(13):2 252-2 259.
[22] 刘月静, 闫杨, 王帅珂, 等. 鼠李糖乳杆菌肠溶性微胶囊的制备研究[J]. 聊城大学学报(自然科学版), 2019, 32(4):34-40.
LIU Y J, YAN Y, WANG S K, et al. Preparation of enteric microcapsulated of Lactobacillus rhamnosus[J].Journal of Liaocheng University (Natural Science Edition), 2019, 32(4):34-40.
[23] RATHER S A, AKHTER R, MASOODI F A, et al.Effect of double alginate microencapsulation on in vitro digestibility and thermal tolerance of Lactobacillus plantarum NCDC201 and L. casei NCDC297 [J]. LWT-Food Science and Technology, 2017, 83:50-58.
[24] 朱莹丹. 植物乳杆菌LIP-1微胶囊制备工艺及其性能的研究[D]. 呼和浩特:内蒙古农业大学, 2015.
ZHU Y D.Study on microencapsulation technologies and their characteristics for Lactobacillus plantarum LIP-1[D]. Hohhot: Inner Mongolia Agricultural University, 2015.
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