Food and Fermentation Industries >

2022 , Vol. 48 >Issue 22: 194 - 200

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030854

Analysis of fungal community structure based on high-throughput sequencing and its correlation with quality indicators of strong-flavor Daqu

  • CHENG Wei ,
  • CHEN Xuefeng ,
  • CHEN Xingjie ,
  • ZHOU Duan ,
  • FU Huan ,
  • XUE Xijia ,
  • LU Jingjing ,
  • DAI Sen
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  • 1(School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China)
    2(Jinzhongzi Distillery Co.Ltd., Fuyang 236023, China)
    3(Shaanxi Research Institute of Agricultural Product Processing Technology, Xi'an 710021, China)

Received date: 2022-01-25

  Revised date: 2022-02-25

  Online published: 2022-12-20

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Abstract

In order to clarify the fungal community structure of strong-flavor Daqu from different regions and its correlation with quality indicators, MiSeq high-throughput sequencing was used to analyze the fungal community structure in surface and inner parts of Daqu from different regions. Saccharification capacity and other quality indicators were detected, and Spearman correlation analysis of fungal community and its quality indicators was taken. The results showed there were 43 fungal operational taxonomic units(OTUs)in common between surface samples, 47 fungal OTUs in inner samples. Rhizopus, Rhizomucor, Blumeria, and Eurotium were the dominant genera in surface samples. While, Thermomyces, Eurotium, and Thermoascus were the dominant genera in inner samples, and their abundances were all account for more than 10%. The correlation analysis showed that there was a significant positive correlation between Thermomyces and moisture, Mortierella and reducing sugar. Rhizopus, Stamphylium and Alternaria were significantly positively correlated with saccharification capacity, and Rasamsonia was significantly positively correlated with esterification capacity. This study has certain reference value for the expansion of Daqu microbiome information.

Key words： high-throughput sequencing; strong-flavor Daqu; fungal diversity; quality indicators; correlation analysis

Cite this article

CHENG Wei , CHEN Xuefeng , CHEN Xingjie , ZHOU Duan , FU Huan , XUE Xijia , LU Jingjing , DAI Sen . Analysis of fungal community structure based on high-throughput sequencing and its correlation with quality indicators of strong-flavor Daqu[J]. Food and Fermentation Industries, 2022 , 48(22) : 194 -200 . DOI: 10.13995/j.cnki.11-1802/ts.030854

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