A solid phase extraction-liquid chromatography-tandem mass spectrometry (SPE-LC/MS) method was established to sim- ultaneously analyze the contents of 14 heterocyclic amines (HAAs) in Jingning traditional braised chicken. This study firstly optimized the instrument parameters of 14 HAAs, and the meat samples were extracted with ethyl acetate and NaOH, purified by PRS and C18 cartridges in series, and eluted with methanol-ammonia (19∶1, V/V).The eluate was mixed and tested on an organic filter.The results showed that the 14 HAAs had good linearity in the concentration range of 1-500 μg/L, the correlation coefficient was not less than 0.999, the detection limit(S/N=3)was 0.06-0.17 μg/kg, the limit of quantification (S/N=10) was 0.19-0.58 μg/kg.The recoveries of 14 HAAs at spiked levels of 20, 50, and 100 μg/L were 85.75%-99.14%, 85.95%-101.17%, 83.04%-103.78%, respectively.The results showed that the method had a wide linear range, high precision and accuracy, short analysis time, and good purification effect, which could meet the requirements of simultaneous detection of multiple HAAs residues in meat products.
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