The preparation of broad-spectrum antibodies and their application in the rapid detection of food safety

  • ZHANG Kaihui ,
  • LIU Yuan ,
  • CUI Yan ,
  • WU Haofen ,
  • XU Yongjun ,
  • MA Ziyu ,
  • JI Yanwei
Expand
  • (College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China)

Received date: 2022-06-29

  Revised date: 2022-08-09

  Online published: 2022-12-20

Abstract

As an old saying goes, food is the paramount necessity of people and food safety is the top priority. In the era of pursuing efficiency, the high-throughput and high-efficiency detection of hazards has become a new trend in the rapid detection of food safety. The immunoassay based on broad-spectrum antibodies provides a new idea for simultaneous and rapid detection of multiple hazards with its ad- vantages of high detection flux, short detection time, less sample consumption, and low total cost. This paper pointed out three main prep- aration methods of broad-spectrum antibodies and their relative merits, analyzed the common strategies to improve the cross-reactivity and affinity of broad-spectrum antibodies, and reviewed their application in the detection of toxins, residues of agricultural and veterinary drugs, and other common food hazards. At the same time, this paper summarized the two main limiting factors of broad-spectrum antibod- ies:preparation blindness and poor detection sensitivity, and finally, looked forward to the development trend of broad-spectrum antibod- ies in the field of food safety.

Cite this article

ZHANG Kaihui , LIU Yuan , CUI Yan , WU Haofen , XU Yongjun , MA Ziyu , JI Yanwei . The preparation of broad-spectrum antibodies and their application in the rapid detection of food safety[J]. Food and Fermentation Industries, 2022 , 48(22) : 311 -318 . DOI: 10.13995/j.cnki.11-1802/ts.032822

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