Higher alcohols are one of the important factors affecting the flavor and drinking quality of Chinese rice wine. A moderate amount of higher alcohols make the rice wine taste full, soft, mellow and harmonious, but high concentration of higher alcohols will lead to fusel flavor and strong intoxication. Overexpression of ALD6 can significantly reduce the production of higher alcohols, but the production of excessive acetic acid inhibits cell growth and metabolism in the Saccharomyces cerevisiae. Since rice wine fermentation is bilateral fermentation, the expression of ALD6 can be regulated by screening suitable sugar-inducible promoters, which can reduce the production of higher alcohols and at the same time weaken its inhibitory effect on cell growth. In this study, six HXT series sugar-inducible promoters Phxt1, Phxt2, Phxt3, Phxt4, Phxt5 and Phxt7 were selected to regulate the expression of acetaldehyde dehydrogenase gene ALD6. The fermentation results of rice wine showed that compared with the control strain a17, the total production of higher alcohols of the inducible recombine strains decreased by 22.29%, 19.26%, 42.56%, 37.84%, 16.72% and 30.74% respectively. In addition, the fermentation results showed that compared with the a17, the constitutive recombine strain a-Ppgk1-A and the inducible recombine strain a-Phxt3-A can significantly reduce the production of total higher alcohols, but the growth of a-Phxt3-A was basically the same as a17.
KANG Xinyue
,
WEI Min
,
JIANG Sen
,
WANG Huan
,
GUO Xuewu
,
XIAO Dongguang
,
WU Xiaole
,
CHEN Yefu
. Regulating ALD6 gene expression by sugar-inducible promoter to reduce the production of higher alcohols in rice wine[J]. Food and Fermentation Industries, 2022
, 48(23)
: 17
-24
.
DOI: 10.13995/j.cnki.11-1802/ts.031054
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