Food and Fermentation Industries >

2022 , Vol. 48 >Issue 23: 34 - 41

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031289

Breeding and fermentation optimization of Rhodobacter sphaeroides with high yield of coenzyme Q10

  • LI Wenxin ,
  • ZEN Weizhu ,
  • ZHOU Jingwen
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  • 1(Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)
    3(Science Center for Future Foods, Jiangnan University, Wuxi 214122, China)

Received date: 2022-02-25

  Revised date: 2022-03-14

  Online published: 2023-01-05

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Abstract

Coenzyme Q10 (CoQ10) is a light yellow and fat soluble quinone commonly existed in the inner cell intima of animals, plants and microorganisms. It is a natural antioxidant and activator of cell metabolism. In this study, a Rhodobacter sphaeroides that can produce CoQ10 was used as the starting strain. Its initial CoQ10 titer was 136.47 mg/L at the shaking flasks. Through the combination of ARTP mutation and porous plate based high-throughput screening, it was determined that the time of ARTP mutation treatment was 120 s and the lethal rate was 90%. In the initial screening stage of the orifice plate, eight high-yielding strains were obtained from the mutant library of 5 184 strains by high-throughput screening. After re-screening, a high-yielding mutant strain 7p22 was obtained. The CoQ10 production was 158.44 mg/L, which was 16.1% higher than that of the original strain. In addition, after verifying its genetic stability, the carbon source, precursor substances and metal ions were further optimized to improve the production of coenzyme Q10. Finally, the culture conditions of mutant strains were optimized in 5 L fermenter. The CoQ10 production was further increased to 1 640.6 mg/L. The high-throughput screening method combined with the screening factor and the Craven test assay used in this study can achieve simple and synergistic acquisition of high-yielding CoQ10 mutant strains.

Key words： coenzyme Q10; Rhodobacter sphaeroides; ARTP mutagenesis; high-throughput screening; fermentation optimization

Cite this article

LI Wenxin , ZEN Weizhu , ZHOU Jingwen . Breeding and fermentation optimization of Rhodobacter sphaeroides with high yield of coenzyme Q10[J]. Food and Fermentation Industries, 2022 , 48(23) : 34 -41 . DOI: 10.13995/j.cnki.11-1802/ts.031289

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