Relationship between sensory indexes, physicochemical indexes, microbial community and volatile compounds in high-temperature Daqu

  • DENG Can ,
  • GAO Ruijie ,
  • ZHAO Yongwei ,
  • MIAO Lihong ,
  • WANG Miankun ,
  • LIU Pulin ,
  • CHEN Jiahui ,
  • FAN Peiwen
Expand
  • 1(College of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China)
    2(Huibei Baiyunbian Distillery Co.Ltd., Songzi 434200, China)

Received date: 2022-02-24

  Revised date: 2022-03-14

  Online published: 2023-01-05

Abstract

The quality of Daqu was evaluated mainly by sensory indexes. In order to explore the difference in grades of high temperature Daqu, superior high-temperature Daqu (SHD) and common high-temperature Daqu (CHD) were taken as models and compared based on sensory characteristics. It was found that whether sensory characteristics have some correlativity with physiochemical indexes, microbial community and volatile compounds. Based on the above, some conclusions could be made: the acidity, ammonia nitrogen content, fermentation power and esterification power of SHD were higher than that of CHD. The water content, liquefaction power and saccharifying power of SHD were lower than that of CHD. The number of culturable bacteria and molds in CHD was higher than those in SHD, and the difference was significant. Diversity of bacterial community in SHD was more abundant, and Bacillus and Saccharopolyspora were the dominant bacteria. Species of fungal community of the two kinds of Daqu were the same, the proportion of Aspergillus in CHD was 18.24%, and that in SHD was 0.57% and the proportion of thermophilic fungi reached 98.89%. A total of 23 volatile compounds were detected in high-temperature Daqu. The contents of isovaleric acid, ethyl hexanoate, furfural and tetramethylpyrazine in SHD were higher than those in CHD, while the contents of isobutyraldehyde and isovaleraldehyde in SHD were lower than those in CHD. This study can provide theoretical basis for sensory evaluation of Daqu quality and reference for the establishment of high-temperature Daqu quality standard.

Cite this article

DENG Can , GAO Ruijie , ZHAO Yongwei , MIAO Lihong , WANG Miankun , LIU Pulin , CHEN Jiahui , FAN Peiwen . Relationship between sensory indexes, physicochemical indexes, microbial community and volatile compounds in high-temperature Daqu[J]. Food and Fermentation Industries, 2022 , 48(23) : 78 -85 . DOI: 10.13995/j.cnki.11-1802/ts.031269

References

[1] 邱并生. 混菌发酵对白酒液态发酵效率和风味物质的影响[J].微生物学通报, 2014, 41(7):1 477-1 478.
QIU B S.Effect of mixed culture on fermentation efficiency and flavor compounds in Chinese liquor[J].Microbiology China, 2014, 41(7):1 477-1 478.
[2] 李静心, 王艳丽, 何宏魁, 等.基于高通量测序技术解析高温大曲和中高温大曲的真菌群落结构[J].食品与发酵工业, 2018, 44(12):52-59.
LI J X, WANG Y L, HE H K, et al.High-throughput sequencing revealed fungal community structures at high temperature Daqu and medium temperature Daqu[J].Food and Fermentation Industries, 2018, 44(12):52-59.
[3] 庄名扬. “浓酱结合型”白酒生产中几个值得注意的问题[J].酿酒科技, 2003(2):22-23.
ZHUANG M Y.Some problems in the production of “Luzhou-flavor & Maotai-flavor combined” liquor[J].Liquor-Making Science & Technology, 2003(2):22-23.
[4] LIU P H, ZHANG L H, DU X W, et al.Dynamic analysis of physicochemical and biochemical indices and microbial communities of light-flavor Daqu during storage[J].Journal of the American Society of Brewing Chemists, 2019, 77(4):287-294.
[5] LI P, LIN W F, LIU X, et al.Effect of bioaugmented inoculation on microbiota dynamics during solid-state fermentation of Daqu starter using autochthonous of Bacillus, Pediococcus, Wickerhamomyces and Saccharomycopsis[J].Food Microbiology, 2017, 61:83-92.
[6] ZHENG X W, YAN Z, NOUT M J R, et al.Microbiota dynamics related to environmental conditions during the fermentative production of Fen-Daqu, a Chinese industrial fermentation starter[J].International Journal of Food Microbiology, 2014, 182-183:57-62.
[7] SHANQIMUGE, LIANG H Z, ZHANG C X, et al.A DGGE marker-mediated fast monitoring of bacterial diversity and comprehensive identification of high-temperature Daqu starter[J].Journal of Food Science, 2015, 80(7):M1519-M1525.
[8] 王玉霞, 李兵, 申孟林, 等.浓香型白酒大曲在发酵和成熟过程中主要功能酶活力分析[J].食品工业科技, 2018, 39(11):270-274;286.
WANG Y X, LI B, SHEN M L, et al.Activities analysis of main functional enzymes from strong-flavour Daqu of Chinese liquor during fermentation and storage stages[J].Science and Technology of Food Industry, 2018, 39(11):270-274;286.
[9] 董大伟, 王晓慧, 苏葛, 等.中高温大曲存储后的感官质量研究[J].酿酒科技, 2018(12):39-44.
DONG D W, WANG X H, SU G, et al.Research on sensory quality of medium/high-temperature Daqu after storage[J].Liquor-Making Science & Technology, 2018(12):39-44.
[10] 敖宗华, 陕小虎, 沈才洪, 等.国内主要大曲相关标准及研究进展[J].酿酒科技, 2010(2):104-108.
AO Z H, SHAN X H, SHEN C H, et al.Domestic related quality standards of Daqu & its research progress[J].Liquor-Making Science & Technology, 2010(2):104-108.
[11] 邢钢, 敖宗华, 邓波.大曲中微生物研究和检测进展[J].酿酒科技, 2012(12):86-89.
XING G, AO Z H, DENG B.Research & detection progress of microbes in Daqu[J].Liquor-Making Science & Technology, 2012(12):86-89.
[12] 宋瑞滨, 邵泽良, 宋军.浓香型大曲贮存过程中部分理化指标及微生物变化[J].酿酒科技, 2017(1):59-61.
SONG R B, SHAO Z L, SONG J.Change in physiochemical indexes and microbial quantity of Nongxiang Daqu during the storage process[J].Liquor-Making Science & Technology, 2017(1):59-61.
[13] 沈才洪, 应鸿, 许德富, 等.大曲的理化特征指标探讨[J].酿酒科技, 2005(9):20-22.
SHEN C H, YING H, XU D F, et al.Study on Daqu quality standards (IV): Investigation on physiochemical property indexes of Daqu[J].Liquor-Making Science & Technology, 2005(9):20-22.
[14] 周斐成, 吴生文, 朱庆圣, 等.特香型大曲发酵过程中曲块不同部位理化指标及主要酶系动态分析[J].中国酿造, 2019, 38(4):58-64.
ZHOU F C, WU S W, ZHU Q S, et al.Physicochemical indexes and dynamic analysis of main enzymes in different layers of Te-flavor Daqu during fermentation process[J].China Brewing, 2019, 38(4):58-64.
[15] 徐岩. 现代白酒酿造微生物学[M].北京:科学出版社, 2019.
XU Y.Modern Baijiu Brewing Microbiology[M].Beijing:Science Press, 2019.
[16] 葛媛媛, 姚粟, 刘洋, 等.芝麻香型白酒高温大曲微生物总 DNA 提取的改良方法[J].酿酒, 2012, 39(4):33-37.
GE Y Y, YAO S, LIU Y, et al.A modified method for DNA extraction from high temperature Daqu of Zhima-flavor Chinese liquor[J].Liquor Making, 2012, 39(4):33-37.
[17] 李豆南, 邱树毅.酱香大曲微生物菌群结构及微生物功能研究概述[J].中国酿造, 2017, 36(1):5-11.
LI D N, QIU S Y.Overview of microbial community structure and function in Moutai-flavor Daqu[J].China Brewing, 2017, 36(1):5-11.
[18] 苏俊霞. 镇江香醋醋醅中醋酸菌多样性及Gluconacetobacter intermedius特性的研究[D].无锡:江南大学, 2014.
SU J X.Biodiversity of acetic acid bacteira in the vinegar Pei of Zhenjiang aromatic vinegar and the propetries of Gluconacetobacter intermedius[D].Wuxi:Jiangnan University, 2014.
[19] 郎召伟. 泸型酒酿造过程中风味物质变化分析[D].无锡:江南大学, 2015.
LANG Z W.Analysis of flavor compounds in the production of Luzhou-flavor liquor[D].Wuxi:Jiangnan University, 2015.
[20] ZHANG W X, WU Z Y, ZHANG Q S, et al.Combination of newly developed high quality Fuqu with traditional Daqu for Luzhou-flavor liquor brewing[J].World Journal of Microbiology and Biotechnology, 2009, 25(10):1 721-1 726.
[21] 李显, 蔡琼慧, 吴凤智.影响酱香型大曲糖化力的因素[J].酿酒科技, 2017(4):79-81;87.
LI X, CAI Q H, WU F Z.Influencing factors of saccharifying power of Jiangxiang Daqu[J].Liquor-Making Science & Technology, 2017(4):79-81;87.
[22] YU H, ZHANG R Y, YANG F W, et al.Control strategies of pyrazines generation from Maillard reaction[J].Trends in Food Science & Technology, 2021, 112:795-807.
Outlines

/