Effects of choline chloride/lactic acid deep eutectic solvent pretreatment on the physicochemical properties and enzymatic hydrolysis efficiency of bamboo shoot shell

  • HU Qiang ,
  • WANG Yanyun ,
  • JIANG Qiong ,
  • GONG Weihua ,
  • WANG Yan
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  • 1(Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, Leshan Normal University, Leshan 614000, China)
    2(College of Life Science, Leshan Normal University, Leshan 614000, China)
    3(National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia ulmoides, Jishou University,Jishou 416000, China)

Received date: 2022-07-13

  Revised date: 2022-08-12

  Online published: 2023-01-05

Abstract

Bamboo shoot husk is a by-product OF bamboo shoot processing. In order to solve the conversion and utilization of bamboo shoot husk biomass, the key step is to establish a green and efficient pretreatment method to improve the enzymatic hydrolysis efficiency of bamboo shoot husk. In this study, bamboo shoot shell was used as raw material and pretreated with deep eutectic solvent (DES) under different conditions. Scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy were used to analyze the composition and physical and chemical characteristics of lignocellulose from bamboo shoot shell. The effect and potential reasons of DES pretreatment on the improvement of cellulase hydrolysis efficiency of bamboo shoot husk samples were explored. The results showed that after DES pretreatment, the cellulose content in sand shoot shell samples increased from 35.86% to 48.63%, the hemicellulose content decreased from 19.13% to 15.59%, and the lignin content decreased from 28.67% to 14.82%. DES pretreatment can effectively destroy the dense appearance of lignocellulose and reduce the anti-degradation barrier of bamboo shoot shell to a large extent. DES greatly reduced lignin and a small amount of hemicellulose, but highly retained cellulose, and DES pretreatment did not change the crystal structure of cellulose. The enzymatic hydrolysis rate of bamboo shoot shell samples increased from 29.24% (untreated) to 90.43% (110 ℃, 1∶9), which was 3.1 times higher than that of raw materials. In summary, choline chloride/lactic acid can achieve efficient enzymatic hydrolysis of bamboo shoot husk under the best conditions (110 ℃, 1∶9), and effectively remove the lignin in bamboo shoot husk.

Cite this article

HU Qiang , WANG Yanyun , JIANG Qiong , GONG Weihua , WANG Yan . Effects of choline chloride/lactic acid deep eutectic solvent pretreatment on the physicochemical properties and enzymatic hydrolysis efficiency of bamboo shoot shell[J]. Food and Fermentation Industries, 2022 , 48(23) : 187 -193 . DOI: 10.13995/j.cnki.11-1802/ts.032963

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