By optimizing the pretreatment conditions (sonication time, solid phase extraction column, eluent type and eluent volume), a high-performance liquid chromatography-fluorescence detector-secondary array tube detector method for the detection of 16 polycyclic aromatic hydrocarbons (PAHs) residues in kebabs was established. acetonitrile and acetonitrile-saturated N-hexane were used as extracts, and the samples were extracted by ultrasound for 30 min, solid phase extraction via polycyclic aromatic hydrocarbons molecular imprinted column (PAH-MIP), elute with 9 mL of n-hexane-dichloromethane (30:70, v/v), the acenaphthene was measured by a diode array detector, and the other 15 PAHs were measured by a fluorescence detector, the chromatographic column was an Agilent SB C18 column, the injection volume was 5 μL, the column temperature was 30 ℃, and the flow rate was 0.8 mL/min., acetonitrile and water were used as mobile phases, and the elution mode was gradient elution. in the range of 1-50 ng/mL, the 16 PAHs had a good linear relationship, and the correlation coefficients were all greater than 0.99. At three spiking levels of 2, 5 and 20 ng/mL, the average recoveries were 81%-104%, the relative standard deviations were 0.95%-5.84%, and the method detection limit (S/N=3) was 0.33-3.30 μg/kg, the limit of quantification (S/N=10) was 1.0-10.0 μg/kg. High-performance liquid chromatography-fluorescence detector-two-stage array tube detection method is suitable for the analysis of PAHs in mutton skewers.
ZHANG Yongsheng
,
HAN Jinhua
,
TIAN Zhu
,
ZHANG Dan
,
ZHANG Zhen
,
SHI Xixiong
. Determination of 16 polycyclic aromatic hydrocarbons in kebabs by high performance liquid chromatography-fluorescence detector-secondary array tube detector[J]. Food and Fermentation Industries, 2022
, 48(23)
: 274
-280
.
DOI: 10.13995/j.cnki.11-1802/ts.030814
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