Food and Fermentation Industries >

2022 , Vol. 48 >Issue 24: 172 - 180

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033349

Alleviating effects of Rosa roxburghii Tratt polysaccharides and flavonoids on mice with ulcerative colitis

  • YUAN Meng ,
  • TAN Shuming ,
  • REN Tingyuan ,
  • XU Qing ,
  • TAN Yunyun ,
  • CHEN Zhen ,
  • TAO Yun ,
  • PU Xian
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  • 1(College of Brewery and Food Engineering, Guizhou University, Guiyang 550025, China)
    2(Guizhou Roxburgh Rose Research Institute, Guiyang 550025, China)

Received date: 2022-08-16

  Revised date: 2022-09-07

  Online published: 2023-01-06

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Abstract

To investigate the intervention effect and mechanism of Rosa roxburghii Tratt total polysaccharide (RP) and R. roxburghii Tratt total flavonoids (RF) in ulcerative colitis (UC) mice with induced by 3% dextran sodium sulfate (DSS). Forty-eight male mice (C57BL/6J) were randomly divided into control group, model group, RP group and RF group, with 12 mice in each group. Except control group, mice in other groups were given 3%DSS solution freely, and were given intragastric administration from the 4th day of modeling for continuous intervention for 10 days. Calculated the disease activity index (DAI). The length of the colon in mice, serum lipopolysaccharide (LPS), D-lactic acid (D-lac) levels, tumour necrosis factor-α(TNF-α), interleukin-1β(IL-1β), interleukin-6(IL-6), interleukin-10(IL-10), myeloperoxidase (MPO), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the colon tissue of UC mice were determined. Pathological changes in colonic tissue and the intestinal barrier were detected. Quantitative real-time polymerase chain reaction and Western blotting were used to detect the relative expression of relevant genes mRNA and protein in the colon NF-κB signaling pathway. Compared with the model group, the RP and RF groups significantly reduced the DAI of colitis mice, protected the pathological damage of colon tissue, improved oxidative stress damage in colitis mice, and reduced the secretion of inflammatory factors. RP and RF reduced inflammatory infiltration and impaired intestinal barrier in mice with colitis, significantly inhibiting the activation of the NF-κB signaling pathway. RP and RF had a relieving effect on DSS-induced UC mice, where the effect of RP was superior to that of RF.

Key words： Rosa roxburghii Tratt polysaccharide (RP); Rosa roxburghii Tratt flavonoid (RF); ulcerative colitis; inflammatory factors; NF-κB signaling pathway

Cite this article

YUAN Meng , TAN Shuming , REN Tingyuan , XU Qing , TAN Yunyun , CHEN Zhen , TAO Yun , PU Xian . Alleviating effects of Rosa roxburghii Tratt polysaccharides and flavonoids on mice with ulcerative colitis[J]. Food and Fermentation Industries, 2022 , 48(24) : 172 -180 . DOI: 10.13995/j.cnki.11-1802/ts.033349

References

[1] 邓代霞, 李凤兰, 李潮云, 等.鱼腥草对葡聚糖硫酸钠诱导溃疡性结肠炎小鼠的缓解及保护作用[J/OL].食品科学,2022.http://kns.cnki.net/kcms/detail/11.2206.TS.20220414.1438.122.html.
DENG D X, LI F L, LI C Y, et al.Palliative and protective effects of Houttuynia cordata on mice with ulcerative colitis induced by dextran sulfate sodium[J/OL].Food Science, 2022.http://kns.cnki.net/kcms/detail/11.2206.TS.20220414.1438.122.html.
[2] NIU W, CHEN X Q, XU R L, et al.Polysaccharides from natural resources exhibit great potential in the treatment of ulcerative colitis:A review[J].Carbohydrate Polymers, 2021, 254:117189.
[3] 吴苹, 刘晋倩, 陈芳.天然多糖对溃疡性结肠炎的治疗作用概述[J].生物学教学, 2021, 46(11):2-4.
WU P, LIU J Q, CHEN F.Summary of the therapeutic effect of natural polysaccharides on ulcerative colitis[J].Biology Teaching, 2021, 46(11):2-4.
[4] KANG J H, CHOI S, JANG J E, et al.Wasabia japonica is a potential functional food to prevent colitis via inhibiting the NF-κB signaling pathway[J].Food & Function, 2017, 8(8):2 865-2 874.
[5] 薛明松, 郑玉玉, 张宇峰, 等.黄连粗多糖协同小檗碱改善溃疡性结肠炎肠黏膜屏障损伤的作用[J].中国实验方剂学杂志, 2022,28(13):71-76.
XUE M S, ZHENG Y Y, ZHANG Y F, et al.Coptidis rhizoma crude polysaccharide and berberine synergistically restore intestinal mucosal barrier damage in ulcerative colitis[J].Chinese Journal of Experimental Traditional Medical Formulae, 2022, 28(13):71-76.
[6] UNGARO R, MEHANDRU S, ALLEN P B, et al.Ulcerative colitis[J].Lancet (London, England), 2017, 389(10 080):1 756-1 770.
[7] CHEN C, TAN S M, REN T Y, et al.Polyphenol from Rosa roxburghii Tratt fruit ameliorates the symptoms of diabetes by activating the P13K/AKT insulin pathway in db/db mice[J].Foods (Basel, Switzerland), 2022, 11(5):636-636.
[8] JI J C, ZHANG S, TANG L, et al.Integrative analysis of fecal metabolome and gut microbiota in high-fat diet-induced hyperlipidemic rats treated with Rosa roxburghii Tratt juice[J].Journal of Functional Foods, 2022, 90:104978.
[9] NI H Y, YU L, ZHAO X L, et al.Seed oil of Rosa roxburghii Tratt against non-alcoholic fatty liver disease in vivo and in vitro through PPARα/PGC-1α-mediated mitochondrial oxidative metabolism[J].Phytomedicine: International Journal of Phytotherapy and Phytopharmacology, 2022, 98:153919.
[10] 周宏炫, 黄颖, 谭书明, 等.刺梨多酚对急性酒精中毒大鼠的解酒和护肝作用[J].食品科学, 2021, 42(17):163-169.
ZHOU H X, HUANG Y, TAN S M, et al. Anti-alcoholic and hepatoprotective effects of polyphenols from the fruit of Rosa roxburghii Tratt in rats with acute alcoholism[J].Food Science, 2021, 42(17):163-169.
[11] 杨笙, 谭书明, 陈超, 等.刺梨多酚的纯化工艺及体外抗氧化研究[J].食品与发酵科技, 2022, 58(1):7-14.
YANG S, TAN S M, CHEN C, et al.Study on purification technology and in vitro antioxidant activity of Rosa roxburghii polyphenols[J].Food and Fermentation Science & Technology, 2022, 58(1):7-14.
[12] 涂永丽, 谭书明, 周宏炫, 等.刺梨口服液对消化不良小鼠胃肠动力的促进作用[J].现代食品科技, 2021,37(1):17-23.
TU Y L, TAN S M, ZHOU H X, et al.Effect of Rose roxburghii Tratt oral liquid on gastrointestinal motility in dyspepsia mice[J].Modern Food Science and Technology, 2021,37(1):17-23.
[13] ZENG F, GE Z, LIMWACHIRANON J, et al.Antioxidant and tyrosinase inhibitory activity of Rosa roxburghii fruit and identification of main bioactive phytochemicals by UPLC-Triple-TOF/MS[J].International Journal of Food Science & Technology, 2017,52(4):897-905.
[14] 王振伟, 郑黎静.刺梨多糖的提取纯化及生物活性研究进展[J].农业技术与装备,2021(11):105-107.
WANG Z W, ZHENG L J.Research progress on extraction purification and biological activity of prickly pear polysaccharide[J].Agricultural Technology & Equipment, 2021(11):105-107.
[15] 孙红艳, 戚晓阳, 王国辉, 等.不同处理对刺梨黄酮含量及其抑菌活性的影响[J].食品研究与开发, 2016,37(5):1-4.
SUN H Y, QI X Y, WANG G H,et al.Influence of different treatment on the total flavonoid content and its antibacterial activity in the fruits of Rosa roxburghii Tratt[J].Food Research and Development, 2016,37(5):1-4.
[16] 张潘, 汪磊, 陈洁, 等.刺梨多糖对非酒精性脂肪肝小鼠回肠粘膜屏障功能的影响[J/OL].食品科学, 2022. http://kns.cnki.net/kcms/detail/11.2206.TS.20220621.1730.051.html.
ZHANG P, WANG L, CHEN J, et al.Effects of Rosa roxburghii Tratt polysaccharide on ileal mucosal barrier function in mice with non-alcoholic fatty liver[J/OL].Food Science, 2022. http://kns.cnki.net/kcms/detail/11.2206.TS.20220621.1730.051.html.
[17] 詹继红, 刘铭洁, 郭银雪.刺梨黄酮抗氧化应激抑制模型大鼠TGF-β1、TGF-βRⅠ延缓肾间质纤维化的实验研究[J].中华中医药学刊, 2018,36(6):1 291-1 293.
ZHAN J H, LIU M J,GUO Y X, et al.Experimental study of Rosa roxburghii flavones oxidation stress inhibition rats′ TGF-β1 and TGF-βRⅠ delaying renal interstitial fibrosis[J].Chinese Archives of Traditional Chinese Medicine, 2018,36(6):1 291-1 293.
[18] 陈超, 谭书明, 王画, 等.刺梨及其活性成分对2型糖尿病小鼠糖脂代谢的影响[J].食品科学,2022,43(13):146-154.
CHEN C, TAN S M, WANG H, et al.Effects of Rosa roxburghii Tratt fruit and its active ingredients on glucose and lipid metabolism in type 2 diabetic mice[J].Food Science,2022,43(13):146-154.
[19] YE Y J, LU Y S, ZHOU X, et al.Protective effects of acupuncture intervention against damage of intestinal mucosal barri er induced by 5-fluorouracil in rats[J].Acupuncture Research, 2016,41(2):95-99.
[20] ZHANG Y K, LU F, ZHANG H, et al.Polysaccharides from Agaricus blazei Murrill ameliorate dextran sulfate sodium-induced colitis via attenuating intestinal barrier dysfunction[J].Journal of Functional Foods, 2022,92:105072.
[21] CHEN Z X, YI L, PAN Y N, et al.Lactobacillus fermentum ZS40 ameliorates inflammation in mice with ulcerative colitis induced by dextran sulfate sodium[J].Frontiers in Pharmacology, 2021,12:700217.
[22] CHEN X Y, LI X, SUN-WATERHOUSE D, et al.Polysaccharides from Sargassum fusiforme after UV/H2O2 degradation effectively ameliorate dextran sulfate sodium-induced colitis[J].Food & function, 2021, 12(23):11 747-11 759.
[23] LI S,CHEN Y,ZHANG Y, et al.Polyphenolic extracts of coffee cherry husks alleviated colitis-induced neural inflammationvia NF-κB signaling regulation and gut microbiota modification[J].Journal of Agricultural and Food Chemistry, 2022,70(21):6 467-6 477.
[24] 王翠芳, 王特日格乐, 杜红喜, 等.沙葱总黄酮水洗组分的体外抗炎活性[J].食品科学,2019,40(23):163-169.
WANG C F, WANG T, DU H X, et al.Anti-inflammatory activity in vitro of water-eluted fraction of total flavonoids extracted from Allium mongolicum Regel[J].Food Science, 2019,40(23):163-169.
[25] 陈国伟, 邱春红, 田灵敏, 等.食源性天然产物中多糖干预炎症性肠病的研究进展[J].食品科学, 2019,40(13):281-287.
CHEN G W, QIU C H, TIAN L M, et al.Recent progress in food-derived natural polysaccharide intervention in inflammatory bowel disease[J].Food Science, 2019,40(13):281-287.
[26] WANG L, ZHANG P, LI C, et al.A polysaccharide from Rosa roxburghii Tratt fruit attenuates high-fat diet-induced intestinal barrier dysfunction and inflammation in mice by modulating the gut microbiota[J].Food & function, 2022,13(2):530-547.
[27] CHEN C H, WANG H, HONG T, et al.Effects of tea polysaccharides in combination with polyphenols on dextran sodium sulfate-induced colitis in mice[J].Food Chemistry:X, 2021,13:100190.
[28] 蒯云逸, 蒋和浩, 红雷, 等.岩藻多糖对大鼠肾脏氧化应激、炎症和细胞凋亡的缓解作用[J].动物营养学报, 2022,34(8):5 393-5 403.
KUAI Y Y, JIANG H H, HONG L, et al.Alleviating effects of fucoidan on oxidative stress, inflammation and apoptosis in kidney of rat[J].Chinese Journal of Animal Nutrition, 2022,34(8):5 393-5 403.
[29] LI S, CHEN Y, ZHANG Y, et al.Polyphenolic extracts of coffee cherry husks alleviated colitis-induced neural inflammation via NF-κB signaling regulation and gut microbiota modification[J].Journal of Agricultural and Food Chemistry,2022, 70(21):6 467-6 477.
[30] GUO X X, XU Y, GENG R X, et al.Curcumin alleviates dextran sulfate sodium-induced colitis in mice through regulating gut microbiota[J].Molecular Nutrition & Food Research, 2022,66(8):e2100943.
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