Schizochytrium sp. is now considered as a promising microorganism to replace marine fish oil for commercial production of DHA-rich lipid. In this study, effects of nitrogen source (yeast extract and monosodium glutamate) and phosphorus source (KH2PO4) concentration on the cell growth, glucose consumption and lipid accumulation in fermentation by Schizochytrium sp. DP-16 were investigated. The fermentation process in control medium containing normal nitrogen and phosphorus source concentration was analyzed. Stage 1 (0-24 h) was cell growth and proliferation stage; stage 2 (24-84 h) was lipid accumulation phase, and the lipid production and lipid content at 84 h reached 14.1 g/L and 33.5% respectively; stage 3 (84-96 h) was lipid turnover stage. Under low KH2PO4 concentration of 1 g/L, the lipid production and lipid content at 72 h reached 14.8 g/L and 44.3% respectively, increased by 5.0% and 32.2% respectively compared with the control group. However, high nitrogen, low nitrogen, high phosphorus and other conditions are not conducive to lipid production. The results showed that the concentration of nitrogen and phosphorus sources had an important effect on the cell growth and lipid accumulation of Schizochytrium sp. Therefore, to control the appropriate concentration of nitrogen and phosphorus source for efficient lipid production, an optimal balance needs to be found between attaining a high concentration of the biomass and a high lipid content in the biomass. This study provides valuable guidance for promoting DHA-rich lipid production by Schizochytrium sp.
FAN Xuening
,
CHENG Yan
,
ZHAO Guofu
,
CHEN Ming
. Effects of nitrogen source and phosphorus source concentration on the growth and lipid accumulation of a strain of Schizochytrium sp.[J]. Food and Fermentation Industries, 2022
, 48(24)
: 189
-194
.
DOI: 10.13995/j.cnki.11-1802/ts.031211
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