Lactobacillus plantarum WUH3 was a lactic acid bacterium isolated from Chinese traditional pickled cucumber, which has good antibacterial activity. In this paper, the properties and application value of L. plantarum WUH3 were studied by gene analysis and experimental verification. Firstly, the whole genome of L. plantarum WUH3 was sequenced and analyzed to predict its properties. Results showed that this strain contained many transporter genes related to bacteriocin synthesis, and three bacteriocins synthesis genes: Plantarin_EF, Pediocin and Enterocin_X_chain_beta, indicating that the strain had the ability to synthesize and secrete bacteriocin. A series of experiments were designed to show that this strain could secrete bacteriocin with the molecular weight between 4.1-6.5 kDa. Its bacteriocin had good thermal stability (121 ℃, 30 min) and pH stability (pH 2-11). Further research showed that this bacteriocin could inhibit a variety of spoilage bacteria and pathogens, which proved it has a broad-spectrum antibacterial activity. Especially, the bacteriocin exerted its antibacterial effect by acting on the surface of cell membrane and causing membranolysis. Finally, we analyzed the safety of L. plantarum WUH3, and found that it was harmless to human body. Thus, it can be used for food preservation. This study is useful for the development and application of L. plantarum WUH3.
GUO Lidan
,
HE Wenqian
,
LI Hongbiao
,
LIN Yi
,
SHA Shenfei
,
WANG Liping
. Analysis of bacteriocin synthesis gene and bacteriocin properties of Lactobacillus plantarum WUH3[J]. Food and Fermentation Industries, 2022
, 48(24)
: 195
-202
.
DOI: 10.13995/j.cnki.11-1802/ts.031514
[1] SU D, LIANG L, LI Z, et al.Genome analysis of two Lactobacillus plantarum strains, LLY-606 and pc-26, for evaluating their potential as probiotics[J].Journal of Microbiology and Biotechnology, 2019.DOI:10.1101/841163.
[2] TREJO-GONZÁLEZ L, GUTIÉRREZ-CARRILLO A E, RODRÍGUEZ-HERNÁNDEZ A I, et al.Bacteriocins produced by LAB isolated from cheeses within the period 2009-2021:A review[J].Probiotics and Antimicrobial Proteins, 2022,14(2):238-251.
[3] HUANG Y Y, LIU D M, JIA X Z, et al.Whole genome sequencing of Lactobacillus plantarum DMDL 9010 and its effect on growth phenotype under nitrite stress[J].LWT, 2021, 149:111778.
[4] SIEZEN R J, TZENEVA V A, CASTIONI A, et al.Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches[J].Environmental Microbiology, 2010, 12(3):758-773.
[5] 李萍, 龙春昊, 赵轩, 等.Ⅱ类细菌素的生物合成及其在食品领域的应用[J].中国食品学报, 2021, 21(10):269-286.
LI P, LONG C H, ZHAO X, et al.Biosynthesis of class Ⅱ bacteriocin and its application in food preservation[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(10):269-286.
[6] PAL G, SRIVASTAVA S.Inhibitory effect of plantaricin peptides (Pln E/F and J/K) against Escherichia coli[J].World Journal of Microbiology and Biotechnology, 2014, 30(11):2 829-2 837.
[7] HANNY E L L, MUSTOPA A Z, BUDIARTI S, et al.Efficacy, toxicity study and antioxidant properties of plantaricin E and F recombinants against enteropathogenic Escherichia coli K1.1 (EPEC K1.1)[J].Molecular Biology Reports, 2019, 46(6):6 501-6 512.
[8] SUN Z H, HARRIS H M B, MCCANN A, et al.Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera[J].Nature Communications, 2015, 6(1):1-13.
[9] CHOKESAJJAWATEE N, SANTIYANONT P, CHANTARASAKHA K, et al.Safety assessment of a nham starter culture Lactobacillus plantarum BCC9546 via whole-genome analysis[J].Scientific Reports, 2020, 10(1):10241.
[10] TENEA G N, ORTEGA C.Genome characterization of Lactiplantibacillus plantarum strain UTNGt2 originated from theobroma grandiflorum (white cacao) of ecuadorian amazon:Antimicrobial peptides from safety to potential applications[J].Antibiotics(Basel,Switzerland), 2021, 10(4):383.
[11] BARBOSA J, ALBANO H, SILVA B, et al.Characterization of a Lactiplantibacillus plantarum R23 isolated from arugula by whole-genome sequencing and its bacteriocin production ability[J].International Journal of Environmental Research and Public Health, 2021, 18(11):5 515.
[12] LUO R B, LIU B H, XIE Y L, et al.Erratum:SOAPdenovo2:An empirically improved memory-efficient short-read de novo assembler[J].GigaScience, 2012, 1(1):18.
[13] ZHAO S M, HAN J Z, BIE X M, et al.Purification and characterization of plantaricin JLA-9:A novel bacteriocin against Bacillus spp.produced by Lactobacillus plantarum JLA-9 from Suan-tsai, a traditional Chinese fermented cabbage[J].Journal of Agricultural and Food Chemistry, 2016,64(3):2 754-2 764.
[14] TICHACZEK P S, NISSEN-MEYER J, NES I F, et al.Characterization of the bacteriocins curvacin a from Lactobacillus curvatus LTH1174 and sakacin P from L.sake LTH673[J].Systematic and Applied Microbiology, 1992, 15(3):460-468.
[15] 张瑜. LS-8乳酸菌细菌素的分离纯化[D].杨凌:西北农林科技大学, 2013.
ZHANG Y.The purification and partial characteriaztion of bacteriocin produced by Lactobacillus rhamnosus LS-8[D].Yangling:Northwest A&F University, 2013.
[16] YI L H, QI T, HONG Y, et al.Screening of bacteriocin-producing lactic acid bacteria in Chinese homemade pickle and dry-cured meat, and bacteriocin identification by genome sequencing[J].LWT, 2020, 125:109177.
[17] XU C, FU Y Y, LIU F, et al.Purification and antimicrobial mechanism of a novel bacteriocin produced by Lactobacillus rhamnosus 1.0320[J].LWT, 2021, 137:110338.
[18] YI L H, DANG J, ZHANG L H, et al.Purification, characterization and bactericidal mechanism of a broad spectrum bacteriocin with antimicrobial activity against multidrug-resistant strains produced by Lactobacillus coryniformis XN8[J].Food Control, 2016, 67:53-62.
[19] WANG Y, QIN Y X, XIE Q, et al.Purification and characterization of plantaricin LPL-1, a novel class Ⅱa bacteriocin produced by Lactobacillus plantarum LPL-1 isolated from fermented fish[J].Frontiers in Microbiology, 2018,9:2276.
[20] TANG W T, ZHANG H, WANG L, et al.New cationic antimicrobial peptide screened from boiled-dried anchovies by immobilized bacterial membrane liposome chromatography[J].Journal of Agricultural and Food Chemistry, 2014, 62(7):1 564-1 571.
[21] JIA F F, ZHANG L J, PANG X H, et al.Complete genome sequence of bacteriocin-producing Lactobacillus plantarum KLDS1.0391, a probiotic strain with gastrointestinal tract resistance and adhesion to the intestinal epithelial cells[J].Genomics,.2017, 109(5-6):432-437.
[22] STERGIOU O S, TEGOPOULOS K, KIOUSI D E, et al.Whole-genome sequencing, phylogenetic and genomic analysis of Lactiplantibacillus pentosus L33, a potential probiotic strain isolated from fermented sausages[J].Frontiers in Microbiology, 2021, 12:746659.
[23] YANG R, JOHNSON M C, RAY B.Novel method to extract large amounts of bacteriocins from lactic acid bacteria[J].Applied and Environmental Microbiology, 1992, 58(10):3 355-3 359.
