This study aimed to establish a rapid and accurate detection system for identifying Panax ginseng, Panax notoginseng, and Panax quinquefolius by multiplex PCR and apply it to detect commercial samples. The selected single nucleotide polymorphism (SNP) sites specific to Panax ginseng, Panax notoginseng, and Panax quinquefolius were used to design primers, locked nucleic acid (LNA) modification and base mismatch were carried out at the 3' end of the primer to close the end and enhance the specificity of the primer. The multiplex PCR detection system was established and the reaction conditions were optimized. The commercial samples were verified. The multiplex PCR detection system was established, and the specific bands of ginseng, Panax ginseng, Panax notoginseng, and Panax quinquefolius were 249, 366, and 212 bp respectively. At the annealing temperature of 59 ℃, 35 cycles, 40 ng of DNA, and the primer ratio of Panax ginseng, Panax notoginsen, and Panax quinquefolius was 0.15 μL∶1.25 μL∶0.3 μL, the reaction conditions were the best. The detection of different reference products showed that the positive rate of rough-processed products was higher than that of deep-processed products. Rough products from different sources in the market were selected for multiplex PCR detection, and the positive rate reached over 80%. A multiplex PCR detection system for Panax ginseng, Panax notoginseng, and Panax quinquefolium was established, which could be used for the detection of commercial samples.
WANG Xiangjun
,
LIU Moyi
,
LI Ying
,
WANG Minghui
,
FAN Xinyuan
,
WANG Tianqi
,
LIU Limei
. Establishment and application of multiplex PCR method for identification of Panax ginseng, Panax notoginseng, and Panax quinquefolius[J]. Food and Fermentation Industries, 2022
, 48(24)
: 288
-293
.
DOI: 10.13995/j.cnki.11-1802/ts.030208
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