To explore the development process of stems and leaves of Panax notoginseng-Polygonatum kingianum composite product, the composition and content of soluble total sugar, saponins, and polyphenols of stems and leaves of Panax notoginseng-Polygonatum kingianum instant powder were analyzed, and its antioxidant activity was determined. A single factor-response surface experiment was conducted to investigate the optimum extraction conditions including ethanol concentration (%), solid-liquid ratio (g∶mL), and extraction time (h) of stems and leaves Panax notoginseng-Polygonatum kingianum. Further, instant powder of stems and leaves of Panax notoginseng-Polygonatum kingianum was obtained by spray drying technology. The total soluble sugar, saponins, and polyphenol contents in the instant powder were determined by ultraviolet spectrophotometry or high-performance liquid chromatography, then the antioxidant activity was determined and sensory evaluation was carried out. The optimal mass ratio of stems and leaves of Panax notoginseng to Polygonatum kingianum by spray drying was 6∶4. Extraction process was as follows: 374.23 g instant powder could be obtained from 1 kg stems and leave of Panax notoginseng-Polygonatum kingianum which extracted by 29% (volume fraction) ethanol with 1∶21 ofsolid-liquid ratios (g∶mL) for 37 h, repeated twice again. The acquisition rates of instant powder were 37.42% with good solubility and sweety within fragrant. The total soluble sugar contents in the instant powder were (55.44±3.21) mg/g. The active components were mainly seven saponins, such as notoginsenoside Fa, Fd, Fc, and ginsenoside Rb1 and five phenolic compounds such as caffeic acid (CA), epicatechin-3-gallate (ECG), and epigallocahechin-3-gallate (EGCG). It was shown that the stems and leaves of Panax notoginseng-Polygonatum kingianum instant powder had good scavenging capacity for ABTS cationic radicals, DPPH free radicals, superoxide anions, and hydroxyl radicals, and the IC50 values were 0.30, 0.63, 0.49, and 1.67 mg/mL, respectively. The extraction process model of stems and leaves of Panax notoginseng-Polygonatum kingianum was optimized, and the instant powder was rich and full of saponins, polyphenols, and soluble sugar. This experiment provided basic research for the stem and leaves of Panax notoginseng and Polygonatum kingianum in the food development field, andprovide scientific support to the development and utilization of characteristic food or drug-food homologous products of Yunnan province.
LIANG Zhengwei
,
WANG Xiaoman
,
LIU Xiaosu
,
ZHANG Shuangyan
,
MA Yan
,
CHEN Junwen
,
ZHANG Guanghui
,
JIANG Weiwei
,
ZHAO Yinhe
,
ZHAO Ming
. Extraction technology and antioxidant activity analysis of stem and leaves of Panax notoginseng-Polygonatum kingianum instant powder[J]. Food and Fermentation Industries, 2023
, 49(2)
: 91
-98
.
DOI: 10.13995/j.cnki.11-1802/ts.030025
[1] WANG T, GUO R X, ZHOU G H, et al.Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) F.H.Chen:A review[J].Journal of Ethnopharmacology, 2016,188:234-258.
[2] CHEN W, KUI L, ZHANG G H, et al.Whole-genome sequencing and analysis of the Chinese herbal plant Panax notoginseng[J].Molecular Plant, 2017, 10(6):899-902.
[3] 习峰敏, 刘昌铭, 李付名, 等.双液相萃取法测定三七块根与茎叶中三七总皂苷含量[J].吉林医药学院学报, 2010, 31(1):24-27.
XI F M, LIU C M, LI F M, et al.Determination of content of total saponins in Panpax notoginseng roots and leaves by aqueous two-phase system[J].Journal of Jilin Medical College, 2010, 31(1):24-27.
[4] CAI S X, LIN J Y.Forgotten treasure-Panax notoginseng stems and leaves[J].Pharmacy Information, 2018, 7(6):141-143.
[5] 施扬, 王力川, 马占林, 等.滇黄精提取物成分与活性测定及细胞毒性评价[J].日用化学工业, 2020, 50(11):788-792;798.
SHI Y, WANG L C, MA Z L, et al.Determination of components and activity of extractions of Polygonatum kingianum and evaluation of its cytotoxicity[J].China Surfactant Detergent & Cosmetics, 2020, 50(11):788-792;798.
[6] 韩凌. 三七叶皂苷活性成分的化学研究[D].沈阳:辽宁中医药大学, 2007.
HAN L.Chemical analysis of saponins active components of stems and leaves of Panax notoginseng[D]. Shenyang:Liaoning University of Traditional Chinese Medicine, 2007.
[7] LE MINH H, DO THI THANH H, NFO THI P, et al.Polygonatum kingianum rhizome extract alleviates collagen antibody-induced arthritis by modulating proinflammatory cytokine production in mice[J].Asian Pacific Journal of Tropical Biomedicine, 2020, 10(11):490-495.
[8] LI R S, TAO A E, YANG R M, et al.Structural characterization, hypoglycemic effects and antidiabetic mechanism of a novel polysaccharides from Polygonatum kingianum Coll.et Hemsl[J].Biomedicine & Pharmacotherapy, 2020,131:110687.
[9] GU W, WANG Y F, ZENG L X, et al.Polysaccharides from Polygonatum kingianum improve glucose and lipid metabolism in rats fed a high fat diet[J].Biomedicine & Pharmacotherapy, 2020, 125:109910.
[10] WANG Q Y, DONG X, YANG J, et al.Vesicle based ultrasonic-assisted extraction of saponins in Panax notoginseng[J].Food Chemistry, 2020, 303:125394.
[11] WEN M C, HAN Z S, CUI Y Q, et al.Identification of 4-O-p-coumaroylquinic acid as astringent compound of Keemun black tea by efficient integrated approaches of mass spectrometry, turbidity analysis and sensory evaluation[J].Food Chemistry, 2022, 368:130803.
[12] GUO X J, ZHANG X L, FENG J T, et al.Purification of saponins from leaves of Panax notoginseng using preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction chromatography[J].Analytical and Bioanalytical Chemistry, 2013, 405(10):3 413-3 421.
[13] KANG J P, HUO Y, YANG D U, et al.Influence of the plant growth promoting Rhizobium panacihumi on aluminum resistance in Panax ginseng[J].Journal of Ginseng Research, 2021,45(3):442-449.
[14] NIAN B, CHEN L J, YI C, et al.A high-performance liquid chromatography method for simultaneous detection of 20 bioactive components in tea extracts[J].Electrophoresis, 2019, 40(21):2 837-2 844.
[15] XIA G H, LI X H, ZHANG Z, et al.Effects of fermentation treatments on Polygonatum odoratum flavones' antioxidant activities[J].Saudi Journal of Biological Sciences, 2021,28(9):5 011-5 016.
[16] SOBULSKA M, ZBICINSKI I.Advances in spray drying of sugar-rich products[J].Drying Technology, 2021, 39(12):1 774-1 799.
[17] YANG X X, WEI J D, MU J K, et al.Integrated metabolomic profiling for analysis of antilipidemic effects of Polygonatum kingianum extract on dyslipidemia in rats[J].World Journal of Gastroenterology, 2018, 24(48):5 505-5 524.
[18] YANG X X, WANG X, SHI T T, et al.Mitochondrial dysfunction in high-fat diet-induced nonalcoholic fatty liver disease:The alleviating effect and its mechanism of Polygonatum kingianum[J].Biomedicine & Pharmacotherapy, 2019,117:109083.
[19] TOHDA C, MATSUMOTO N, ZOU K, et al.Abeta (25-35)-induced memory impairment, axonal atrophy, and synaptic loss are ameliorated by M1, A metabolite of protopanaxadiol-type saponins[J].Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology, 2004,29(5):860-868.
[20] WANG X S, YANG L, YANG L, et al.Gypenoside IX suppresses p38 MAPK/Akt/NFκB signaling pathway activation and inflammatory responses in astrocytes stimulated by proinflammatory mediators[J].Inflammation, 2017, 40(6):2 137-2 150.
[21] LIU J J, JIANG C Y, MA X, et al.Notoginsenoside Fc attenuates high glucose-induced vascular endothelial cell injury via upregulation of PPAR-γ in diabetic Sprague-Dawley rats[J].Vascular Pharmacology, 2018, 109:27-35.
[22] LAI Y Q, TAN Q X, XV S, et al.Ginsenoside Rb1 alleviates alcohol-induced liver injury by inhibiting steatosis, oxidative stress, and inflammation[J].Frontiers in Pharmacology, 2021, 12:616409.
[23] 朱玉昌,焦必宁.ABTS法体外测定果蔬类总抗氧化能力的研究进展[J].食品与发酵工业, 2005, 31(8):77-80.
ZHU Y C, JIAO B N.Research progress on ABTS assay in determination of TAA in fruits and vegetables in vitro[J].Food and Fermentation Industries, 2005, 31(8):77-80.
[24] JIE Z S, LIU J, SHU M C, et al.Detection strategies for superoxide anion:A review[J].Talanta, 2022, 236:122892.
