Advances in heterologous expression and application of α-glucosidase

  • LI Linbo ,
  • ZHANG Shishuang ,
  • YANG Tianyou ,
  • WANG Baoshi ,
  • ZHANG Mingxia
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  • (School of Life Science and Technology, Henan Institute of Science and Technology, Henan Collaborative Innovation Center in Modern Biological Breeding, Xinxiang 453003, China)

Received date: 2022-04-21

  Revised date: 2022-04-30

  Online published: 2023-02-15

Abstract

α-Glucosidase is currently mainly used for the preparation of isomaltooligo-saccharide in industry, because of its functions of hydrolysis of glycosidic bonds and transfer of glycosyl. At the same time, it shows great application potential in the synthesis of glycoconjugates with biological functions, showing broad application prospects in cosmetics, food, medicine, and other fields. In this paper, the characteristics and heterologous expression of α-glucosidases from different sources are reviewed. The industrial synthesis pathway of α-glucosidases for IMOs and the application potential of α-glucosidases in the synthesis of glycoside bioactive substances are discussed. The development direction of molecular modification of α-glucosidases was prospected, which provides a reference for obtaining mutants of glycoside synthases that meet special needs.

Cite this article

LI Linbo , ZHANG Shishuang , YANG Tianyou , WANG Baoshi , ZHANG Mingxia . Advances in heterologous expression and application of α-glucosidase[J]. Food and Fermentation Industries, 2023 , 49(2) : 325 -332 . DOI: 10.13995/j.cnki.11-1802/ts.032053

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