The industrial fermentation process is facing some bottlenecks in data management, data analysis and process optimization control. The rapidly-developing information technology provides a powerful tool for the industrial fermentation process in breaking through the technical barriers. The deep integration with informatization is the only way to improve the competitiveness for the fermentation industry. In order to train high-level technical talents with a cross-disciplinary background required by the deep integration of informatization and fermentation industry, the course Intelligent Control Technology of Fermentation Process is set for the college students majoring in bioengineering. The lecturing contents include building of python virtual environment, collection and management of fermentation data, embedded development and fermentation equipment control, data and control command transmission, and examples of comprehensive application. Aiming at the bioengineering students’ weak foundation in information technology, the course reorganizes the knowledge system according to the requirements of intelligent control of fermentation process. The teaching contents are closely related to the actual application of fermentation engineering and explain the application methods of information technology in the field of fermentation engineering through the familiar cases. At the same time, this course focuses on cultivating students′ motivation for continuous learning and self-learning ability, helps students form a cross disciplinary thinking mode, and improves their ability to solve practical engineering problems by using information technology and fermentation engineering technology.