Acetaldehyde with high toxicity, mutagenicity and carcinogenicity exists widely in food and environment, which threatened human health. Aldehyde dehydrogenase can catalyze the conversion of various aliphatic and aromatic aldehydes into corresponding acids, thereby reducing the hazard of aldehydes, especially acetaldehyde. To obtain a high-producing acetaldehyde dehydrogenase strain from grapes, disulfiram resistance screening combined with enzyme activity assay were used, and the acetaldehyde degradation ability and enzymatic properties of acetaldehyde dehydrogenase were studied. Combining morphological features and ITS rRNA gene sequence, its species was identified. The acetaldehyde dehydrogenase activity was calculated according to the change in the absorbance of NADH at 340 nm, and its acetaldehyde degradation ability was determined by headspace-gas chromatography. The strain was identified as Candida tropicalis and named it as LBBE-W1. The crude enzyme solution of strain LBBE-W1 was extracted by ultrasonic and purified to obtain Candida tropicalis acetaldehyde dehydrogenase (CTALDH) with a specific enzyme activity of 181.17 U/mg. The optimum temperature of CTALDH was 60 ℃ and the optimum pH was 8.0-9.0. It showed good stability after being endured for 2 h at 30-60 ℃ and pH 5.0-9.0. The metal ions Na+, K+ and Ba2+ can obviously promote the activity of CTALDH, while Ni2+, Cu2+ and Mn2+ showed strong inhibitory effect on its activity. Acetaldehyde is the most suitable substrate, and the degradation rate of acetaldehyde by CTALDH reaches 95.12% within 2 h. In this study, a strain of Candida tropicalis LBBE-W1 was obtained, which produced acetaldehyde dehydrogenase with high activity, stability and acetaldehyde degradation capacity, offering the possibility of acetaldehyde degradation in food and environment.
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