Lactobacillus plantarum microcapsules can increase the resistance of the bacteria to adverse conditions, thereby reducing their loss of activity. Microcapsules prepared by traditional methods have problems such as uneven particle size distribution and irregular shape, while microfluidic technology has the characteristics of precise control of the size and shape of microcapsules, and can improve microencapsulation output through highly arrayed microchannels. In this paper, array chips integrating multiple MFDGs were designed. The effects of two fluid distribution layer structures and two droplet generator modes on droplet generation were compared. The droplet diameter, coefficient of variation and droplet generation frequency were used as evaluation indicators. The results showed that the pressure between the microchannels distributed in the tree-like distribution is more balanced, and the fluid can be transported more uniformly; the circular array is more conducive to the formation of monodisperse droplets. The circular array chip with tree-like distribution was selected for the embedding of L. plantarum. When the flow rate ratio was 15, the droplet generation frequency was 20.3 Hz, and the embedding rate was 96.4%, which achieved high-efficiency encapsulation and controlled release of probiotics. This study provides a new idea for high-throughput preparation of L. plantarum microcapsules.
ZHU Zuoyin
,
PENG Tian
,
ZHOU Xinli
. High-throughput microfluidic array chip design and study on preparation of Lactobacillus plantarum microcapsules[J]. Food and Fermentation Industries, 2023
, 49(3)
: 125
-130
.
DOI: 10.13995/j.cnki.11-1802/ts.032205
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