An analytical method based on high-performance liquid chromatography-ultraviolet detector (HPLC-UVD) was established to determine the residues of copper abietate in mango, pitaya, and cowpea. The sample was extracted with hydrochloric acid and petroleum ether, which was ultrasonicated. The extracted supernatant was evaporated to dryness, and then dissolved with acetonitrile, the HPLC-UVD was used to quantitatively determine the sample. The copper abietate in the samples was acidified with hydrochloric acid and changed to abietic acid and the abietic acid was the target for the quantitation of copper abietate. Another high-resolution mass spectrometer was used to qualitatively analyze the copper abietate derivative. Results showed that the correlation coefficient R2 was 0.999 6 for copper abietate, the recovery of copper abietate in the selected fruit and vegetable samples was in the range of 77%-108%, and the relative standard deviations of copper abietate in the three fruits and vegetables, pitaya, cowpea, and mango were less than 5%. These results indicated that the accuracy of the copper rosin acid analysis method in fruits and vegetables met the requirements of detection and analysis. The limit of detection (LOD) for copper abietate was 0.03 mg/kg and the limit of quantitation (LOQ) was 0.1 mg/kg. The method is simple, accurate, and fast for the determination of residue of copper abietate in fruits and vegetables, where the sensitivity and accuracy can meet the requirements of copper abietate residue detection in fruits and vegetables.
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