Peptide AKRA(Ala-Lys-Arg-Ala)was used to treat LO2 cells and alcoholic liver injury mice respectively. In vivo and in vitro study of AKRA on protecting LO2 from oxidative stress and alleviating liver injury caused by alcohol in mice. LO2 cells were divided into normal group and AKRA treatment group. The cell viability was measured by CCK8. AAPH (400 μmol/L) is employed to induce oxidative stress in LO2 cells. The cells were treated with AKRA (1, 3, and 6 mg/mL). The contents of superoxide dismutase (SOD), catalase (CAT), glutathione reduction (GSH), and malondialdehyde (MDA) in cell lysate were detected. The oxidation related marker proteins, autophagy related marker proteins, and inflammatory related marker proteins were evaluated by Western blotting. Mice with liver injury induced by alcohol were treated with different dose of AKRA for 30 d. The mice were killed and the pathomorphological changes of liver were observed and the physiological and biochemical indexes and oxidation related indexes in liver were detected. Compared with the alcohol group, the liver tissue of AKRA treatment group showed less mild degeneration of hepatocytes, decrease of ALT, AST, and ALP levels, downregulating the levels of TC, TG, and LDL, and increasing HDL in serum. In liver tissue, AKRA increased the activity of CAT, GSH, and SOD and decreased the content of MDA. Thus, peptide AKRA may alleviate liver injury via upregulating antioxidation, improving autophagy activity and anti-inflammatory ability in liver cells.
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