When ferulate esterase reacts with lignocellulose, it is difficult to completely separate the free enzyme from the product or residual solid substrate, which increases the production cost. However, feruloyl esterase was covalently immobilized onto a pH responsive polymer Eudragit L-100 with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) as cross-linking agent, which could solve the problem. The parameters of immobilized enzyme such as crosslinking agent concentration, immobilization pH, immobilization time, and reaction temperature were optimized. Under the optimum conditions (EDC 5 g/L, pH 6.0, immobilization time 1 h, and immobilization temperature 25 ℃), the immobilized enzyme exhibited much better thermal stability compared with free enzyme. After 1 h of incubation at 40 ℃, the residual activity was about 50% and 30% for the immobilized enzyme and free enzyme, respectively. In addition, the immobilized enzyme also exhibited significantly improved pH stability and about 64% residual activity was retained after five runs when using destarched wheat bran as substrate to produce ferulic acid. The pH responsive polymer Eudragit L-100 immobilized enzyme proposed in this study offers an attractive approach for enzymatic hydrolysis of insoluble substrate.
WANG Kaipeng
,
ZHENG Jingying
,
XU Xueyang
,
DING Yiling
,
LYU Yongmei
,
LIU Jinbin
,
YU Xiaohong
,
JIN Wenbin
. pH Responsive polymer Eudragit L-100 immobilized feruloyl esterase for ferulic acid production[J]. Food and Fermentation Industries, 2023
, 49(4)
: 224
-229
.
DOI: 10.13995/j.cnki.11-1802/ts.031779
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