Food and Fermentation Industries >

2023 , Vol. 49 >Issue 4: 304 - 311

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031773

Research progress on the biosynthesis of glucosamine and N-acetyl glucosamine

  • LIU Xinlu ,
  • CHEN Fengqianrui ,
  • ZHANG Jiaqi ,
  • LI Jiancheng ,
  • WANG Pengchao
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  • 1(Aulin College, Northeast Forestry University, Harbin 150040, China)
    2(School of Life Science, Northeast Forestry University, Harbin 150040, China)

Received date: 2022-03-31

  Revised date: 2022-04-20

  Online published: 2023-03-20

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Abstract

Glucosamine (GlcN) forms a chemical compound when the second hydroxyl group of glucose is substituted with an amino group. GlcN has become common in organisms including animals, plants, and microorganisms. It is also a main component of the polysaccharides like chitin and chitosan. GlcN and N-acetylglucosamine (GlcNAc), play an important role in inhibiting inflammation and improving skin moisture, especially in treatment for osteoarthritis. As a result, they have been widely applied in the health care and cosmetics industries. Currently, the main methods for GlcN production involve chemical extraction and enzymatic catalysis which hydrolyzes shells of shrimp or crab. However, these methods can lead to severe environmental pollution, high costs, and low efficiency. With the development of metabolic engineering and synthetic biology, GlcN synthesized by microbial fermentation has the advantages of mild production conditions, high product purity, short production cycle, and fewer regional and seasonal restrictions, which have been widely used. We introduce methods of enhancing the main GlcN pathway and blocking the branch pathway by modifying key enzymes, the application of dynamic regulatory tools of ribozyme and sRNA, and the application of directed evolution strategies of strain genomes in the synthesis of GlcN and GlcNAc. This review summarizes the latest progress and future development prospects of GlcN and GlcNAc production.

Key words： biosynthesis; glucosamine; N-acetylglucosamine; microbial fermentation; biosynthetic pathway

Cite this article

LIU Xinlu , CHEN Fengqianrui , ZHANG Jiaqi , LI Jiancheng , WANG Pengchao . Research progress on the biosynthesis of glucosamine and N-acetyl glucosamine[J]. Food and Fermentation Industries, 2023 , 49(4) : 304 -311 . DOI: 10.13995/j.cnki.11-1802/ts.031773

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