Chitooligosaccharides (COS) is an oligosaccharide with various biological activities. To explore the effects of COS and its monomer components (degrees of polymerization 2-4) on insulin resistance of hepatocytes, an insulin-resistant HepG2 cell model was established by insulin induction. The results showed that COS significantly enhanced the glucose consumption in insulin resistant HepG2 cells, suggesting that it promoted glucose metabolism of those cells. Further evaluation of the monomer components of COS exhibited that chitotriose obviously promoted the glucose consumption in insulin resistant HepG2 cells, while chitobiose and chitosan could not significantly show that ameliorative effect. Further mechanism analysis illustrated that COS and chitotriose increased the protein levels of insulin receptor (IR), insulin receptor substrate 1 (IRS-1), glucose transporter 4 (GLUT4) and activated protein kinase B (PKB/Akt), while it improved the transcription level of related genes as well. Conclusively, COS improved the insulin resistant HepG2 cells by activating Akt/GLUT4 pathway, among which chitotriose showed excellent performance.
LIU Peng
,
LI Heng
,
GONG Jinsong
,
JIANG Min
,
XU Hongyu
,
XU Zhenghong
,
SHI Jinsong
. Ameliorative effect and mechanism of chitooligosaccharides on insulin resistance of HepG2 cells[J]. Food and Fermentation Industries, 2023
, 49(5)
: 32
-37
.
DOI: 10.13995/j.cnki.11-1802/ts.032124
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