Food and Fermentation Industries >

2023 , Vol. 49 >Issue 5: 53 - 59

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031927

Screening of effective fraction from Eriobotrya japonica (Thunb.) Lindl with inhibiting activity against α-glucosidase and α-amylase and its inhibition kinetics

  • WEI Aihong ,
  • LI Xiaohong ,
  • ZENG Huang ,
  • LIANG Shuhe ,
  • LIN Zhanwen ,
  • ZHUANG Yuanbei ,
  • ZHANG Shengyuan
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  • 1(Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Jiaying University, Meizhou 514015, China)
    2(Institute of Hakka Medicinal Bio-resources, Medical College, Jiaying University, Meizhou 514031, China)
    3(Central Laboratory of Molecular Biology, Medical College, Jiaying University, Meizhou 514031, China)

Received date: 2022-04-11

  Revised date: 2022-05-06

  Online published: 2023-04-06

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Abstract

To compare the inhibitory activities of alcohol extracts from different medicinal parts (roots, stems, leaves, flowers, pulp, seeds) of Eriobotrya japonica(Thunb.) Lindl on α-glucosidase and α-amylase, and to explore the enzymatic activity of the strongest active parts and their total flavonoids Reaction kinetic characteristics. The alcoholic extracts of different medicinal parts of E. japonica were prepared by ultrasonic extraction with 95% ethanol, and the total flavonoids were prepared by ultrasonic-assisted extraction and AB-8 macroporous resin. The inhibitory activities of α-glucosidase and α-amylase were determined by ultraviolet spectroscopy. The type of enzymatic inhibition was inferred by enzymatic kinetics method and Lineweaver-Burk curve. The results showed that the alcohol extracts from different medicinal parts of E. japonica had certain α-glucosidase and α-amylase inhibitory activities, and the order of α-glucosidase inhibitory activity was flower>stem>root>leaf>pulp>seeds, the order of α-amylase inhibitory activity was root>stem>flower>leaf>pulp>seed. The α-glucosidase inhibitory activity IC50 values of E. japonica flower alcohol extract and E. japonica flower total flavonoids were (4.65±0.35) g/L and (0.017 4±0.003 5) μg/mL, all of which were reversible and non-competitive inhibition types; The IC50 values of α-amylase inhibitory activity were (14.41±0.59) g/L and (1.57±0.03) g/L, both of which were reversible and non-competitive inhibition types. The E. japonica root had the strongest inhibitory activity on α-amylase, with IC50 value of (1.51±0.24) g/L, which was a reversible competitive inhibition type. The results of this study provide a scientific basis for the development and utilization of loquat as a hypoglycemic food and drug.

Key words： Eriobotrya japonica (Thunb.)Lindl; α-glucosidase; α-amylase; enzyme reaction kinetics; Lineweaver-Burk

Cite this article

WEI Aihong , LI Xiaohong , ZENG Huang , LIANG Shuhe , LIN Zhanwen , ZHUANG Yuanbei , ZHANG Shengyuan . Screening of effective fraction from Eriobotrya japonica (Thunb.) Lindl with inhibiting activity against α-glucosidase and α-amylase and its inhibition kinetics[J]. Food and Fermentation Industries, 2023 , 49(5) : 53 -59 . DOI: 10.13995/j.cnki.11-1802/ts.031927

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