Xylanase has a wide range of applications in papermaking, winemaking, etc. To obtain xylanase with low cost and high enzymatic activity, Aronia melanocarpa (Michx.) Elliott was used as the main medium material. The culture conditions for the co-fermentation of Trichoderma reesei and Trichoderma viride in Aronia melanocarpa for xylanase production were investigated through single-factor and orthogonal experiments. The results showed that the culture conditions with the highest xylanase activity were: 0.5 g/L NH4NO3 as the nitrogen source, 25% pomace (mass fraction), T. reesei∶T. viride 3∶2(mass ratio), an inoculation mass fraction of 14%, pH 5.50. Under the optimized conditions, the xylanase activity was as high as 121.62 U/mL on the fourth day of incubation. Additionally, the influence of different MgSO4, FeSO4, MnSO4, ZnCl2 ,and CoCl2 concentrations on the activity of xylanase were studied. The effect of Mg2+ and Mn2+ on the production of xylanase from the co-fermentation of T. reesei and T. viride was higher than that of Co2+, Fe2+ and Zn2+. Therefore, the optimal addition of inorganic ions was 12 mg/L MgSO4 and 1.4 mg/L MnSO4. Combining the optimal culture conditions from the orthogonal experiment and the optimal addition of inorganic ions, the xylanase activity was as high as (127.25±0.09) U/mL, an increase of 69.46%, compared to the basal medium. The co-fermentation of T. reesei and T. viride in Aronia melanocarpa to produce xylanase had low cost and high enzyme yield, which provided a valuable reference for the industrial production of xylanase.
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