To understand the distribution of chitinase in Litopenaeus vannamei and the effect of ultraviolet-C (UV-C) irradiation on chitinase, the chitinase activity in various tissues of L. vannamei and the process of UV-C irradiation and its effects on the characteristics of chitinase were studied. The results showed that the specific activity of chitinase in the liver, stomach, intestine, head shell, shell, and tail of L. vannamei were (72.84±2.54), (33.54±1.31), (27.25±0.91), (21.40±1.25), (10.74±0.48), and (5.22±0.05) U/g raw material, respectively. Chitinase was mainly distributed in shrimp heads (including liver, stomach, and head shell), accounting for 84.83%. The optimal process conditions for UV-C irradiating chitinase in the orthogonal experiment were: irradiation power of 10 W, irradiation time of 25 min, and irradiation height of 10 cm, under which the specific activity of chitinase was (27.06±0.10) U/g raw material. The chitinase in different tissues was activated to different degrees under the optimal process. The optimum temperature of chitinase before and after irradiation in shrimp heads was 40 ℃, and the optimum pH was changed from 4 to 5. Mn2+, Zn2+, Fe3+, and Cu2+ inhibited the chitinase before and after irradiation significantly. In conclusion, the chitinase after UV-C irradiation has the potential to produce chitosan, which provides a reference for UV-C irradiation to improve the chitinase activity in by-products of L. vannamei.
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