Food and Fermentation Industries >

2023 , Vol. 49 >Issue 6: 111 - 119

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034086

Ameliorative effect and mechanism of durian shell polysaccharide on dextran sodium sulfate-induced colitis in mice

  • GUAN Guoqiang ,
  • WANG Yuhui ,
  • XU Xiaotian ,
  • DUAN Xiaoqun
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  • 1(School of Pharmacy, Guilin Medical University, Guilin 541199, China)
    2(School of Biomedical Industry, Guilin Medical University, Guilin 541199, China)
    3(Industrial Technology Research Institute, Guilin Medical University, Guilin 541199, China)

Received date: 2022-10-27

  Revised date: 2022-12-08

  Online published: 2023-04-14

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Abstract

This paper used dextran sodium sulfate (DSS) to establish a mouse model of ulcerative colitis, which was intervened with mesalazine [200 mg/(kg·d)] and durian shell polysaccharide [250 mg/(kg·d), 500 mg/(kg·d), 1 000 mg/(kg·d)] to detect changes in body weight, disease activity index (DAI), and colon length in mice. Myeloperoxidase (MPO) activity in colonic tissue was determined by using an MPO kit. Inflammatory cytokines were evaluated by ELISA. The histopathological changes of the colon were observed by HE staining, and the protective effect of durian shell polysaccharide on ulcerative colitis in mice and its mechanism of action were comprehensively evaluated. Compared with the DSS group, mice in the durian shell polysaccharide high-dose intervention group had increased body weight and colon length, decreased DAI and colon weight, significantly lowered expression levels of pro-inflammatory factors (P<0.01), and significantly lowered inflammatory marker MPO (P<0.01). Compared with the model group, the expression levels of ZO-1, occludin, claudin-7, and E-cadherin in the intestinal tissue of mice in the durian shell polysaccharide group showed a dose-dependent increase (P<0.05) and a dose-dependent decrease (P<0.05) in the expression level of N-cadherin protein, and the colonic epithelial structure was improved. In a cellular model, durian shell polysaccharide repaired mucosal damage and reduced intestinal inflammation by upregulating E-cadherin protein expression levels and downregulating N-cadherin protein expression levels. Durian shell polysaccharide improves DSS-induced intestinal mucosal injury in mice and TNF-α-induced epithelial barrier dysfunction in Caco-2 cells and has good protective effects against ulcerative colitis.

Key words： durian shell polysaccharide; ulcerative colitis; pro-inflammatory factor; intestinal mucosal injury; waste utilization

Cite this article

GUAN Guoqiang , WANG Yuhui , XU Xiaotian , DUAN Xiaoqun . Ameliorative effect and mechanism of durian shell polysaccharide on dextran sodium sulfate-induced colitis in mice[J]. Food and Fermentation Industries, 2023 , 49(6) : 111 -119 . DOI: 10.13995/j.cnki.11-1802/ts.034086

References

[1] CURRÒ D, IANIRO G, PECERE S, et al.Probiotics, fibre and herbal medicinal products for functional and inflammatory bowel disorders[J].British Journal of Pharmacology, 2017, 174(11):1 426-1 449.
[2] YANG T, MA X, WANG R L, et al.Berberine inhibits IFN-γ signaling pathway in DSS-induced ulcerative colitis[J].Saudi Pharmaceutical Journal, 2022, 30(6):764-778.
[3] HOSSEN I, WU H, LUO T, et al.Phytochemicals and inflammatory bowel disease:A review[J].Critical Reviews in Food Science and Nutrition, 2020, 60(8):1 321-1 345.
[4] TURNER D, RICCIUTO A, LEWIS A, et al.STRIDE-II:An update on the selecting therapeutic targets in inflammatory bowel disease (STRIDE) initiative of the international organization for the study of IBD (IOIBD):Determining therapeutic goals for treat-to-target strategies in IBD[J].Gastroenterology, 2021, 160(5):1 570-1 583.
[5] DU L, HA C.Epidemiology and pathogenesis of ulcerative colitis[J].Gastroenterology Clinics of North America, 2020, 49(4):643-654.
[6] HONG C J, CHEN S Y, HSU Y H, et al.Protective effect of fermented okara on the regulation of inflammation, the gut microbiota, and SCFAs production in rats with TNBS-induced colitis[J].Food Research International (Ottawa, Ont.), 2022, 157:111390.
[7] GUO H R, GUO H, XIE Y, et al.Mo3Se4 nanoparticle with ROS scavenging and multi-enzyme activity for the treatment of DSS-induced colitis in mice[J].Redox Biology, 2022, 56:102441.
[8] ZHAN Y F, HOU X T, FAN L L, et al.Chemical constituents and pharmacological effects of durian shells in ASEAN countries:A review[J].Chinese Herbal Medicines, 2021, 13(4):461-471.
[9] 谢果, 吴敏芝, 成金乐, 等.榴莲皮提取物抗炎作用研究[J].广州中医药大学学报, 2015, 32(1):130-135.
XIE G, WU M Z, CHENG J L, et al.Experimental study of anti-inflammation of durian peel extract[J].Journal of Guangzhou University of Traditional Chinese Medicine, 2015, 32(1):130-135.
[10] 姜慧敏. 榴莲皮多糖调节免疫功能的研发[D].开封:河南大学, 2020.
JIANG H M.Study of immune function regulate of polysaccharides extracted from Durio zibethinus Murr rind[D].Kaifeng:Henan University, 2020.
[11] 宋苗苗. 榴莲皮多糖结构表征及抗凝血活性研究[D].开封:河南大学, 2019.
SONG M M.Structural characterization and anticoagulantactivity of polysaccharide from Durio zibethinus Murr peel[D].Kaifeng:Henan University, 2019.
[12] JIANG H M, DONG J, JIANG S J, et al.Effect of Durio zibethinus rind polysaccharide on functional constipation and intestinal microbiota in rats[J].Food Research International, 2020, 136:109316.
[13] YAN Y X, SHAO M J, QI Q, et al.Artemisinin analogue SM934 ameliorates DSS-induced mouse ulcerative colitis via suppressing neutrophils and macrophages[J].Acta Pharmacologica Sinica, 2018, 39(10):1 633-1 644.
[14] HUANG C Y, LUO X Q, LI L L, et al.Glycyrrhiza polysaccharide alleviates dextran sulfate sodium-induced ulcerative colitis in mice[J].Evidence-Based Complementary and Alternative Medicine:ECAM, 2022, 2022:1345852.
[15] WAN F, WANG M Y, ZHONG R Q, et al.Supplementation with Chinese medicinal plant extracts from Lonicera hypoglauca and Scutellaria baicalensis mitigates colonic inflammation by regulating oxidative stress and gut microbiota in a colitis mouse model[J].Frontiers in Cellular and Infection Microbiology, 2022, 11:798052.
[16] XU X T, WEI C Q, YANG Y Y, et al.New discovery of anti-ulcerative colitis active ingredients of Nostoc commune:P-Hydroxy benzaldehyde[J].Journal of Functional Foods, 2021, 77:104327.
[17] XU X, LUO A, LU X,et al.p-Hydroxybenzoic acid alleviates inflammatory responses and intestinal mucosal damage in DSS-induced colitis by activating ERβ signaling[J].Journal of Functional Foods,2021,87:104835.
[18] LÊ A, MANTEL M, MARCHIX J, et al.Inflammatory bowel disease therapeutic strategies by modulation of the microbiota:How and when to introduce pre-, pro-, syn-, or postbiotics?[J].American Journal of Physiology-Gastrointestinal and Liver Physiology, 2022, 323(6):G523-G553.
[19] WANG J L, ZHANG Q H, DENG Y W, et al.Efficacy and safety of heat-sensitive moxibustion in the treatment of ulcerative colitis:A protocol for a systematic review and meta-analysis[J].Medicine, 2021, 100(4):e24078.
[20] TIAN H C, WEN Z Y, LIU Z C, et al.Comprehensive analysis of microbiome, metabolome and transcriptome revealed the mechanisms of Moringa oleifera polysaccharide on preventing ulcerative colitis[J].International Journal of Biological Macromolecules, 2022, 222(Pt A):573-586.
[21] GUO Y F, LI Y, CAO Q, et al.The function of natural polysaccharides in the treatment of ulcerative colitis[J].Frontiers in Pharmacology, 2022, 13:927855
[22] LARABI A, BARNICH N, NGUYEN H T T.New insights into the interplay between autophagy, gut microbiota and inflammatory responses in IBD[J].Autophagy, 2020, 16(1):38-51.
[23] PAN H H, ZHOU X X, MA Y Y, et al.Resveratrol alleviates intestinal mucosal barrier dysfunction in dextran sulfate sodium-induced colitis mice by enhancing autophagy[J].World Journal of Gastroenterology, 2020, 26(33):4 945-4 959.
[24] FITZPATRICK J A, MELTON S L, YAO C K, et al.Dietary management of adults with IBD—The emerging role of dietary therapy[J].Nature Reviews Gastroenterology & Hepatology, 2022, 19(10):652-669.
[25] WANG Y J, LI Q M, ZHA X Q, et al.Intervention and potential mechanism of non-starch polysaccharides from natural resources on ulcerative colitis:A review[J].International Journal of Biological Macromolecules, 2022, 210:545-564.
[26] MIAO X P, SUN X N, CUI L J, et al.Suppressive effect of pectic polysaccharides extracted from Rauwolfia verticillata (Lour.) Baill.var.hainanensis Tsiang on inflammation by regulation of NF- κ B pathway and interleukin-17 in mice with dextran sulphatesodium-induced ulcerative colitis[J].Asian Pacific Journal of Tropical Medicine, 2015, 8(2):147-152.
[27] ISHISONO K, MANO T, YABE T, et al.Dietary fiber pectin ameliorates experimental colitis in a neutral sugar side chain-dependent manner[J].Frontiers in Immunology, 2019, 10:2979.
[28] TANG S, LIU W, ZHAO Q Q, et al.Combination of polysaccharides from Astragalus membranaceus and Codonopsis pilosula ameliorated mice colitis and underlying mechanisms[J].Journal of Ethnopharmacology, 2021, 264:113280.
[29] 薛明松, 郑玉玉, 张宇峰, 等.黄连粗多糖协同小檗碱改善溃疡性结肠炎肠黏膜屏障损伤的作用[J].中国实验方剂学杂志, 2022, 28(13):71-76.
XUE M S, ZHENG Y Y, ZHANG Y F, et al.Coptidis rhizoma crude polysaccharide and berberine synergistically restore intestinal mucosal barrier damage in ulcerative colitis[J].Chinese Journal of Experimental Traditional Medical Formulae, 2022, 28(13):71-76.
[30] CHEN Y S, LIAN Y Z, CHAO J.Protective effect of Lycium barbarum polysaccharides and capsaicin in rats with dextran sulfate sodium-induced ulcerative colitis via anti-inflammation and antioxidation[J].Current Developments in Nutrition, 2021, 5(Supplement 2):306.
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