Food and Fermentation Industries >

2023 , Vol. 49 >Issue 6: 156 - 161

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.031587

Antibacterial mechanism of cinnamon essential oil against Pseudomonas deceptionensis CM2 isolated from chicken meat

  • LI Bo ,
  • ZHENG Kaixi ,
  • MA Yunfang ,
  • XIANG Qisen
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  • 1(College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China)
    2(Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou University of Light Industry, Zhengzhou 450001, China)

Received date: 2022-03-17

  Revised date: 2022-05-11

  Online published: 2023-04-14

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Abstract

Pseudomonas spp. are recognized as the specific spoilage microorganisms of meat and meat products. It is of great importance to search the safe and efficient natural food preservatives to ensure meat safety. In this work, the minimum inhibitory concentration of cinnamon essential oil against the Pseudomonas deceptionensis CM2 isolated from spoilage chicken was determined by microbroth dilution method. The antibacterial mechanisms of cinnamon essential oil were studied by measuring the cell membrane integrity, cell membrane potential, and intracellular reactive oxygen species level. The results showed that cinnamon essential oil exhibited strong antibacterial activity against P. deceptionensis CM2 with the minimum inhibitory concentration of 0.156 μL/mL. After being treated with cinnamon essential oil at 0.156 μL/mL for 4 h, the extracellular nucleic acids and proteins concentrations were increased by 13.5- and 4.7-fold (P<0.05) as high as that of the control. The fluorescent intensities of propidium iodide and N-phenyl-1-naphthylamine value of P. deceptionensis CM2 cells were increased by 32.6% and 51.9% (P<0.05), respectively. The membrane potential and intracellular reactive oxygen species level of P. deceptionensis CM2 cells were increased by 2.8-fold and 45.0% (P<0.05), respectively. In summary, cinnamon essential oil can effectively inactivate P. deceptionensis CM2 cells, which may be related to the disruption of cell membrane structure and function, and the induction of oxidative damage. The results provide a scientific basis for the practical application of cinnamon essential oil in food preservation.

Key words： cinnamon essential oil; Pseudomonas deceptionensis CM2; antibacterial activity; cell membrane structures

Cite this article

LI Bo , ZHENG Kaixi , MA Yunfang , XIANG Qisen . Antibacterial mechanism of cinnamon essential oil against Pseudomonas deceptionensis CM2 isolated from chicken meat[J]. Food and Fermentation Industries, 2023 , 49(6) : 156 -161 . DOI: 10.13995/j.cnki.11-1802/ts.031587

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